|Table of Contents|

Efficient Regeneration System for Vaccinium uliginosum L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年11
Page:
124-129
Research Field:
Publishing date:

Info

Title:
Efficient Regeneration System for Vaccinium uliginosum L.
Author(s):
LI Bing1; 2; SU Shang2; 3; WANG Lijin2; 3; WU Jie2; 3; WANG Liangsheng2; RAO Liqun1
(1.College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128；2.Institute of Botany,Chinese Academy of Sciences,Beijing 100093；3.University of Chinese Academy of Sciences,Beijing 100049)
Keywords:
Vaccinium uliginosum L.; regeneration; rooting; chlormequat chloride; nursery bag; transplantation
PACS:
-
DOI:
10.11937/bfyy.201711025
Abstract:
The tender stem explants with leaves from Vaccinium uliginosum L.were cultured on medium supplemented with different type,concentration and combination of plant growth regulators to study their effects on adventitious buds regeneration and rooting and shoot growth,respectively.And the effects of different transplanting techniques on the survival rate of plantlets were studied as well.The results indicated that ZT could promote the differentiation of adventitious buds from explants such as leaves and stem segments.The multiplication coefficient was reduced and the adventitious buds were more green and stout when explants were cultured on medium with IBA.The best medium for adventitious buds regeneration was MWPM+1.0 mg?L-1 ZT+0.1 mg?L-1 IBA.Low concentration of IBA could effectively promote the rooting rate of shoots and chlormequat could make the seedlings more strong so that the optimum medium for rooting and strong seedling was MWPM+0.5 mg?L-1 IBA+5.0 mg?L-1 CCC.In the process of transplanting the survival rate reached 100% when the transplanting technique assisted with the nursery bag was used.The results showed that an efficient regeneration system was established for Vaccinium uliginosum L.in this study.

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Last Update: 2017-06-16