|Table of Contents|

Identification of Resistance Gene Sw-5 and Ty-3a by Multiplex PCR in Tomato(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年11
Page:
115-118
Research Field:
Publishing date:

Info

Title:
Identification of Resistance Gene Sw-5 and Ty-3a by Multiplex PCR in Tomato
Author(s):
JIN Fengmei; XUE Jun; SONG Jian; WANG Shu; ZHANG Yue
(Tianjin Research Center of Agricultural Biotechnology,Tianjin 300192)
Keywords:
tomato spotted wilt virus(TSWV); tomato yellow leaf curl virus; Sw-5 gene; Ty-3a gene; multiplex PCR
PACS:
-
DOI:
10.11937/bfyy.201711023
Abstract:
Tomatoes with spotted virus resistant and susceptible genotypes and yellow leaf curl virus resistant and susceptible genotypes were used as test materials.The multiplex PCR system was developed to detect the Sw-5 and Ty-3a gene simultaneously in order to provide a more time-saving,labor-saving and cost-effective method for tomato resistance breeding.The results showed that the amplified fragments by multiplex PCR were identical with single-primer PCR.A 574 bp band was amplified in the resistant genotype,and a 464 bp band was amplified in the susceptible genotype by the SCAR marker of Sw-5 gene.A 630 bp band could be amplified in the resistant genotype and a 320 bp band was amplified in the susceptible genotype by the SCAR marker of Ty-3a gene.The multiplex PCR system could amplify two genes in one reaction.It could be useful marker assisted selection during seedling stage in tomato and efficiently speed up breeding procedure.

References:

 

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Last Update: 2017-06-15