|Table of Contents|

Cloning and Arabidopsis Ectopic Expression of PpSnRK1βλ1 From Prunus persica(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年11
Page:
98-106
Research Field:
Publishing date:

Info

Title:
Cloning and Arabidopsis Ectopic Expression of PpSnRK1βλ1 From Prunus persica
Author(s):
ZHAO Yongfei; CHEN Xiaolu; PENG Futian; LUO Jingjing; ZHAO Xin; XIAO Yuansong
(College of Horticulture Science and Engineering，Shandong Agricultural University/State Key Laboratory of Crop Biology，Tai′an，Shandong 271018)
Keywords:
peach(Prunus persica); PpSnRK1βλ1; gene clone; expression analysis; functional study
PACS:
-
DOI:
10.11937/bfyy.201711021
Abstract:
Wild peach was used as test material，PpSnRK1βλ1，the regulatory subunit βλ of SnRK1 (Sucrose non-fermenting-1-related protein kinase-1)，was cloned and its expressions in different organs was analyzed.And to assay its function，transgenic plants were obtained successfully by transgenic technology.The results indicated that the reading frame of PpSnRK1βλ1 possessed 1 476 bp and encoded 492 amino acid residues deduced from the DNA sequence.PpSnRK1βλ1 contained CBM and four CBS domain.The deduced PpSnRK1βλ1 protein was highly homologous to other PpSnRK1βλ1proteins from different species，and PpSnRK1βλ1 was very closely related to SnRK1βλ of GuoMei.Real-time PCR results showed that the PpSnRK1βλ1 expressed in root，stalk and leaf of peach seedling.The expression level of PpSnRK1βλ1 was very low in stalk of peach seedling.Via statistical studies，the results indicated that transgenic plant flowered 2.19 days later than WT，and produced around 1.17 rosette leaves than WT.The contents of chlorophyll，soluble sugar，and soluble proteins of transgenic plant were significantly higher than WT，which increased by 13.7%，12.9% and 23.3%，respectively.There was no difference between transgenic plant and WT in starch content.Under the condition of oxidative stress，the growth rate of the wild-type Arabidopsis was severely suppressed，while the transgenic Arabidopsis showed less impact.Transgenic plants showed better seed germination rate and relatively longer root length than wild type，which could support plant growth under stress condition.In a word，the results of study indicated that PpSnRK1βλ1 participated in regulating carbon and nitrogen metabolism and had effect on flower time，and played a role in the resistant of oxidative stress.

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Last Update: 2017-06-15