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Effect of Three Biological Stains on Plant Growth and Membrane Lipid  Peroxidation System of Chlorophytum comosum var.variegatum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年11
Page:
75-82
Research Field:
Publishing date:

Info

Title:
Effect of Three Biological Stains on Plant Growth and Membrane Lipid  Peroxidation System of Chlorophytum comosum var.variegatum
Author(s):
ZHANG Yongfu; NIU Yanfen; ZHAO Feng; HAN Li; WANG Dingkang; LI Ying
(School of Agriculture，Kunming University，Kunming,Yunnan 650214)
Keywords:
Chlorophytum comosum var. variegatum; biological stain; plant growth; membrane lipid peroxidation; biomass
PACS:
-
DOI:
10.11937/bfyy.201711017
Abstract:
Chlorophytum comosum var. variegatum was used as test material，the effect of three biological stains (methyl orange，methyl violet and neutral red) of different concentration (0,0.5,1.0,2.0 mmol?L-1) on plant growth and membrane lipid peroxidation system were studied.The results showed that after treatment with the three concentrations of methyl violet and 0.5 mmol?L-1 of methyl orange and neutral red，biomass，root length，leaf length and leaf width of Chlorophytum comosum var. variegatum were significantly larger than those of control (CK)，after 2.0 mmol?L-1 treatment，the above morphological indicators were significantly less than CK，whereas after 1.0 mmol?L-1 treatment and CK were not significantly different.After treatment，MDA，H2O2 content，oxygen free radical production rate and peroxidase (POD) activity of leaves were higher than CK during the whole experiment，and with the increase of concentration and time，there was an obvious upward trend；the superoxide dismutase (SOD) increased first and then decreased with time，but the activity of 2.0 mmol?L-1 treatment was lower than that of low concentration，which indicated that the antioxidant system was beginning to disorder.Thus，combined with the effect of dying agent on plant growth and membrane lipid peroxidation system，1.0 mmol?L-1 methyl orange，methyl violet and neutral red were more suitable for Chlorophytum comosum var. variegatum intravital staining.

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Last Update: 2017-06-15