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Cloning and Sequence Analysis of CsPAP-fib Gene in Cucumber(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年20
Page:
40-45
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of CsPAP-fib Gene in Cucumber
Author(s):
LIU Weifeng; XIN Ming; ZHOU Xiuyan; QIN Zhiwei
College of Horticulture and Architecture Landscape,Northeast Agricultural University,Harbin,Heilongjiang 150030
Keywords:
cucumber; CsPAP-fib gene; clone; sequence analysis
PACS:
-
DOI:
10.11937/bfyy.20171060
Abstract:
The tempetature-sensitive cucumber cultivar ‘C09-123’ was used as material in this study,a protein which concerned the formation of cucumber femaleness under low night temperature had been found by the identification of differential proteins in cucumber young leaves treated by different night temperatures,then was analysed by bioinformatics technology.The gene encoded this protein was cloned by cloning technique to establish a foundation for confirming the functional mechanisms of CsPAP-fib in cucumber sex differentiation,and provide the genetic material for further research.The results showed that the gene length was 870 bp,encoding 289 amino acids.The protein encoded by this gene was an unstable,hydrophobic and non-transmembrane protein without signal peptide located in chloroplast,and it was highly homologous to Cucumis melo L.The gene was named as CsPAP-fib,based on the protein family PAP-fibrillin which the protein encoded by the gene.

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Last Update: 2017-10-31