|Table of Contents|

Optimization of Protocol of Tissue Culture and Rapid Propagation of Pinellia ternata of Protocorm-like Body(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年01
Page:
14-18
Research Field:
Publishing date:

Info

Title:
Optimization of Protocol of Tissue Culture and Rapid Propagation of Pinellia ternata of Protocorm-like Body
Author(s):
LI Xianliang1ZHAO Zhichang2
1.College of Bioengineering,Jingchu University of Technology,Jingmen,Hubei 448000;2.Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Danzhou,Hainan 571737
Keywords:
Pinellia ternataprotocorm-like bodytissue culture and rapid propagationoptimization
PACS:
-
DOI:
10.11937/bfyy.20171032
Abstract:
Jing Pinellia ternata was used as test material,in way of orthogonal test method,the influence of the explants and their sterilization methods on contamination from endophyte was studied.And on this base the influence of the explants and the cytokinin on the induction of protocorm-like body and the method of the transformation from protocorm-like body to seed stems were further studied systematically to provide references for rapid progagation of Pinellia ternata seed stems.The results showed that using the young petoiles with unopened leaves as explants and adding the step of using benzalkonium bromide with the concentration of 0.1% soak the explants for 3 minutes could reduce the contamination rate.The young petoiles inculated on the medium of MS+TDZ 0.5-1.0 mg?L-1 could be better induced into protocorm-like bodies.The protocorm-like bodies inoculated on the medium of 1/3MS+6-BA 0.5 mg?L-1 +NAA 0.1 mg?L-1 could form more clustered plantlets,and then more seed stems.

References:

[1]楼之岑,秦波.常用中药材品种整理和质量研究[M].北京:北京医科大学中国协和医科大学联合出版社,1995:919.

[2]李先端,胡世材,杨边菊,等.半夏类药材氨基酸与无机元素分析[J].中国中药杂志,1990,15(10):37.
[3]国家药典委员会.中国药典一部[M].北京:中国医药科技出版社,2010:110.
[4]李玉先,刘晓东,朱照静.半夏药理作用的研究述要[J].辽宁中医学院学报,2004,6(6):459-460.
[5]张科卫,吴皓,李伟.HPLC同时测定半夏药材中鸟次黄嘌呤核苷、鸟嘌呤核苷的含量[J].药物分析杂志,2005,25(5):487-489.
[6]郑国灿.半夏提取液的抗肿瘤性研究[J].四川中医,2004,22(9):9-11.
[7]张小丽,谢人明,冯英菊.四种中药对血小板聚集性的影响[J].西北药学杂志,2000,15(6):260-261.
[8]蒋文跃,杨宇,李燕燕.化痰药半夏、瓜英、浙贝母、石曹摘对人鼠血液流变性的影响[J].中医杂志,2002,43(3):215-216.
[9]郭巧生.半夏研究进展[J].中药研究与信息,2000,2(10):15-20.
[10]顾德兴,李云香,徐炳声.半夏的繁殖生物学研究[J].植物资源与环境学报,1994,3(4):44-48.
[11]薛建平,朱艳芳,张爱民,等.半夏试管块茎直接再生技术的研究[J].作物学报,2004,30(10):1060-1064.
[12]张爱民,杨生玉,薛建平,等.多种因素对半夏外植体直接诱导形成试管小块茎的影响[J].中国中药杂志,2005,30(8):576-579.
[13]李光胜,张志立,毛文岳.应用组织培养方法系统选育三叶半夏[J].植物学报,1992(1):59.
[14]江艳华,李艾莲,陈彩霞,等.半夏类原球茎的诱导及其植株再生研究[J].中国农学通报,2013,29(19):153-158.
[15]刘建玲,陈宝宝,雷毅,等.半夏产生物碱内生菌的分离及其抑菌活性的初步研究[J].西北植物学报,2010,30(4):645-651.
[16]刘建玲,陈宝宝,刘永红,等.半夏内生菌的分离培养与鉴定[J].中国中药杂志,2009,34(18):2305-2307.
[17]张洁,万芬,李玉权,等.半夏内生细菌的分离与鉴定[J].贵州农业科学,2014,42(3):94-97.

Memo

Memo:
-
Last Update: 2018-01-11