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Separation and Diversity Analysis of Endophytic Fungi From  Lycium barbarum L.in Ningxia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年10
Page:
103-109
Research Field:
Publishing date:

Info

Title:
Separation and Diversity Analysis of Endophytic Fungi From  Lycium barbarum L.in Ningxia
Author(s):
XU Quanzhi; SUN Mudi; LI Fan; GAO Yuan; GU Peiwen
(College of Agriculture，Ningxia University，Yinchuan，Ningxia 750021)
Keywords:
Lycium barbarum L.; endophytic fungi; diversity; rDNA-ITS
PACS:
-
DOI:
10.11937/bfyy.201710024
Abstract:
Lycium barbarum L.was used as test material，endophytic fungi were separated from different varieties and different parts of Lycium barbarum L.by tissue separation.They were classified according to their morphological characteristics of culture，colonies and spores and rDNA-ITS sequences analysis.The composition characteristics of endophytic fungi were analyzed according to their colonization rate(CR)，relative frequency(RF)，shannon-weiner index(H)，margalef index(D) and Sorenson′s similarity coefficient(CS).The results showed that 363 endophytic fungi were isolated from L.barbarum L.，and they belonged to 14 genus，Alternaria，Aspergillus，Bipolaris,Chaetomium,Cladosporium,Fusarium,Penicillium,Preussia,Schizotheciu,Sordaria,Taifanglania,Thielavia,Tricharina,Xylaria，respectively. Alternaria，Thielavia and Aspeirgllus were dominant genus and their relative frequency(RF) were 46%，14% and 13% respectively.These endophytic fungi were distributed in various organs of L.barbarum L.The types of endophytic fungi in the stems were the most 0.86 in these organs of L.barbarum L.Sorenson′s similarity coefficient(CS)of leaf and fruit was the largest.The distribution of endophytic fungi was different between different varieties of Lycium barbarum L.Shannon-wiener index(H) and margalef index(D) of endophytic fungi from cultivated variety of L.barbarum L.，‘Ningqi 1’ ‘Ningqi 2’ were significantly higher than wild varieties，Lycianthes barbatum L.var.auranticarpum K.F.Ching and Lycium ruthenicum Murr.The community composition similarity of endophytic fungi showed that some endophytic fungi had the preference of a certain host and tissue.The endophytic fungi were rich in the resources of endophytic fungi，the endophytic fungi had high host specificity，and their distribution was influenced by the environment.

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Last Update: 2017-06-01