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Development of Nucleic Acid Test Strip for Detection of  Cucumber Green Mottle Mosaic Virus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年10
Page:
100-103
Research Field:
Publishing date:

Info

Title:
Development of Nucleic Acid Test Strip for Detection of  Cucumber Green Mottle Mosaic Virus
Author(s):
HUO Yayun1; 2; ZHANG Yongjiang2; LI Weimin1; ZHU Luoluo3; HU Lin3; YOU Qimin3
(1.Institute of Biotechnology Research，Chinese Academy of Agricultural Sciences，Beijing 100081；2.Chinese Academy of Inspection and Quarantine，Beijing 100176；3.Ustar Biotechnologies (Hangzhou) Co.Ltd.，Hangzhou，Zhejiang 310012)
Keywords:
cucumber green mottle mosaic virus; nucleic acid test strip; detection
PACS:
-
DOI:
10.11937/bfyy.201710023
Abstract:
Positive sample of CGMMV was used as research object.Three primers and two probes were designed according to the coat protein of CGMMV and probes were labeled with Biotin and FAM at 5′ terminus separately.The nucleic acid strip was used to show the detection result after isothermal RT-PCR at the temperature of 59 ℃ for 90 minutes.The other five viruses that also infect cucurbitaceae crops were used to assay the specificity of this method.Diluted nucleic acid solution with certain concentration was used as template to confirm the sensitivity of this method.The results showed that nucleic acid strip was a special method to distinguish CGMMV from the other five plant virus which also infect cucurbitaceae crops，CMV，PMMoV，TMV，ToMV and ZYMV.And the sensibility of this method was 24.300 ng?μL-1 of total RNA.Thus，nucleic acid strip was a rapid，special and sensitive detection method for CGMMV and can be used in routine testing.

References:

 

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Last Update: 2017-06-01