|Table of Contents|

Optimization of Embryoid Regeneration System From ‘Lujia No.5’ Columnar Apple(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年10
Page:
95-99
Research Field:
Publishing date:

Info

Title:
Optimization of Embryoid Regeneration System From ‘Lujia No.5’ Columnar Apple
Author(s):
ZHOU Aiqin1; ZHU Jun1; YANG Qingshan2; WANG Zhenmeng2; LI Yongtao2; WEI Haixia2
(1.Qingdao Agricultural University，Qingdao，Shandong 266109；2.Shandong Academy of Forestry，Jinan，Shandong 250014)
Keywords:
embryoid; regeneration; ‘Lujia No.5’; optimization
PACS:
-
DOI:
10.11937/bfyy.201710022
Abstract:
It is very important to establish a stable genetic transformation system with high frequency stability，which can be used to regenerate plants with the somatic embryogenesis.In this experiment，new variety of apple‘Lujia No.5’was used as test material，the regeneration system of embryoid bodies was studied，which laid the 〖JP〗foundation for the establishment of genetic transformation system.The results showed that MS+6-BA 1.0 mg?L-1+NAA 0.20 mg?L-1 was the most suitable subculture medium.It was considered that the best placed mode was adxial surface touching medium.The best time length of dark culture was 10 days.The best embryo cell media was MS+6-BA 1.0 mg?L-1+NAA 5.0 mg?L-1+TDZ 0.5 mg?L-1.During embryonic development，the embryos in different periods of morphological and histological observation，results showed that the embryoids experienced proembryonal,globular embryo，heart-shaped embryo，torpedo shaped embryo，cotyled onary embryo and mature embryos stages.

References:

 

[1]STEWARD F C,MAPES M O,MEARS K.Growth and organized development of cultured cells.II.Organization in cultures grown from suspended cells[J].Amer f Bot,1958,45:705-708.

[2]朱华国,王红娟,李鹏飞,等.绿色棉新彩棉7号体细胞胚胎发生及其植株再生[J].棉花学报,2013,25(2):110-114.

[3]杨模华,张冬林,李志辉,等.马尾松幼胚体细胞胚胎发生研究[J].植物生理学报,2011,47(9):904-912.

[4]支玉玺,张剑侠,王跃进.华东葡萄广西-2花药胚状体诱导与再生植株的研究[J].果树学报,2010,27(1):18-23.

[5]程钰,孟强,董丽芬,等.长俊木瓜体细胞胚胎诱导的初步研究[J].西北林学院学报,2008,23(6):98-100.

[6]崔广荣,侯喜林,张子学,等.蝴蝶兰叶片离体培养胚状体的发生及组织学观察[J].园艺学报,2007,34(2):431-436.

[7]姚进,黄坚钦,胡恒康,等.香榧体细胞胚发生的初步研究[J].浙江农林大学学报,2013,30(1):129-135.

[8]魏海霞,祝军,刘德玺,等.2苹果品种叶片胚状体再生体系的建立[J].西北林学院学报，2014,29(1):84-88.

[9]雷海英,武擘,贾彦琼,等.大豆体细胞胚胎发生再生体系的建立与优化[J].华北农学报,2012,27(3):29-34.

[10]张波,罗青,张曦,等.宁夏枸杞花药培养胚状体的诱导[J].北方园艺,2016(9):105-108.

[11]郭荣荣.葡萄胚状体再生体系的建立及葡萄天冬氨酸蛋白酶家族基因功能研究[D].杨凌:西北农林科技大学,2015.

[12]陈小鹏,刘拴桃,孙晓镭,等.黄瓜未受精子房的胚状体诱导研究初报[J].西北农业学报,2005,14(2):148-151.

[13]王璐,陈小燕,张力,等.不同因素对黄瓜未受精子房胚状体诱导的影响[J].西北农业学报,2008,17(4):267-270.

[14]达克东,张松,李雅志.苹果叶柄体细胞胚胎发生[J].核农学报,1996,10(2):75-78.

[15]TSUKAHARA K,KOZAKI I,OOMURA M.Adventitious bud formation on apple and quince leaves[J].Japan Breed,1985,35:2-3.

[16]魏爱民,杜胜利,赵晶,等.黄瓜子叶节离体体细胞再生体系研究[J].华北农学报,2011,26(增刊):20-23.

[17]达克东,张松,李雅志.苹果离体叶片培养直接体细胞胚胎发生研究[J].园艺学报,1996,23(3):241-245.

[18]达克东,张松,米瑞芙,等.创伤诱导苹果离体叶片高效直接体细胞胚胎发生[J].核农学报,2001,15(5):290-293.

[19]项伟波.香榧体胚发生体系优化及发育过程的显微观察[D].临安:浙江农林大学,2015.

[20]侯丙凯,鲍雪珍,于惠敏,等.葡萄离体培养中胚状体发生的研究Ⅰ.胚状体发生的组织细胞学观察[J].山东大学学报(自然科学版),1997,32(2):208-212.

[21]PREDIERI S,MALAVASI F.High-frequency shoot regeneration from leaves of the apple rootstock M26[J].Plant Cell,1989（17）:133-142.

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Last Update: 2017-06-01