Buds of Weigela ‘Red Prince’+vat were used as explants.By using tissue culture methods，the optimum medium for Weigela ‘Red Prince’+vat were selected via adding different concentrations of hormone,to provide technique reference for promotion of Weigela‘Red Prince’+vat.The results showed that the inducing culture medium of MS+0.6 mg?L-1 6-BA+0.1 mg?L-1 NAA+sucrose 30 g?L-1+agar 7 g?L-1 was optimal for inducement，the inducement rate was 86.3%;the best proliferation medium was MS+1.0 mg?L-1 6-BA+1.0 mg?L-1 GA3+sucrose 30 g?L-1+agar 7 g?L-1，the proliferation times was 7.250 6;the best rooting medium formula was 1/2MS+1.0 mg?L-1 IBA+sucrose 30 g?L-1+agar 7 g?L-1,the rooting rate was 100.0%.