|Table of Contents|

Technological Study of Processing Pepper Anther Culture in Xinjiang(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年19
Page:
6-10
Research Field:
Publishing date:

Info

Title:
Technological Study of Processing Pepper Anther Culture in Xinjiang
Author(s):
LAI Lili12ZHANG Jiayuan1YANG Mei1SONG Wensheng2ZHU Huaguo1
1.College of Agronomy,Shihezi University,Shihezi,Xinjiang 832000;2.Xinjiang Longping High-Tech Hongan Seeds Co.Ltd.,Shihezi,Xinjiang 832000
Keywords:
pepperanther cultureembryo
PACS:
-
DOI:
10.11937/bfyy.20170971
Abstract:
Six processing pepper varieties 207,208,64,66,67 and TH113 in Xinjiang were used for anther culture.The anthers in different stages were induced in basic MSB medium supplemented with NAA,2,4-D,TDZ and KT in various concentrations.Effects of different hormone treatment on processing pepper anther culture were studied,and established a suitable anther culture method for processing pepper in Xinjiang.The results showed that different ratio of callus induction and embryo induction emerged in different genotype peppers,the ratio of callus induction were 4.98%-29.65% and the ratio of embryo induction were 0-6.88%.0.5 mg?L-1 2,4-D+0.1 mg?L-1 TDZ and 0.5 mg?L-1 NAA+0.1 mg?L-1 KT were the suitable hormone combination for callus induction and embryo induction,respectively.Anther culture conducted before middle of July in Xinjiang was effective.In this study,56 haploid plants were achieved by acclimatization and transplant followed embryo development.

References:

[1]王玉英,孙敬三,王敬驹,等.小黑麦和辣椒花粉植株的诱导率[J].中国科学,1973(1):104-107.

[2]GEORGE L,NARAYANASWAMY S.Haploid capsicum through experimental androgenesis[J].Protoplasm,1973,78:467-470.
[3]张馨宇,王永成,刘爱群,等.辣椒花药培养研究新进展[J].中国农学通报,2007(8):331-334.
[4]李春玲,蒋钟仁.甜辣椒花药培养单倍体育种技术[C]//邹学校.中国辣椒.北京:中国农业出版社,2000:191-205.
[5]KRISTIANSEN K,ANDERSON S B.Effects of donor plant temperature,photoperiod and age on anther culture response of Capsicum anuum L.[J].Euphytica,1993,67:105-109.
[6]张树根,沈火林.辣椒花药培养单倍体育种技术研究进展[J].辣椒杂志,2006(3):1-5.
[7]MITYKO J,ANDRASFALVY A,CSILLERY G,et al.Anther-culture response in different genotypes and F1 hybrids of pepper (Capsicum annuum L.)[J].Plant Breeding,1995,114:78-80.
[8]DUMAS der V R,CHAMBONNET D,POCHARD E.Culture in vitro d′antheres de piment (Capsicum annuum L.):Amelioration destauxd′ obtention de plantes ches differents genotypes par des traitmentsa+35℃[J].Agronomie,1981(1):859-864.
[9]王玉英,李春玲,蒋钟仁.辣椒和甜椒花药培养的新进展[J].园艺学报,1981,8(2):41-45.
[10]张恩让,须海丽.贵州地方辣椒花药培养体系的初步研究[J].辣椒杂志,2005(4):24-27.
[11]赵激,邹学校.不同培养基对辣椒花药培养的影响[J].湖南农业大学学报(自然科学版),2010(4):181-187.
[12]王仲慧,刘金兵,刁卫平,等.辣椒花药培养胚状体发生途径影响因子研究[J].核农学报,2015(8):1471-1478.
[13]付文婷,廖芳芳,何建文,等.不同温度和外源添加物对辣椒花药的培养效果[J].南方农业学报,2015(4):635-640.

Memo

Memo:
-
Last Update: 2017-10-11