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Separation of Allelochemicals of the Sixth Chromatography Fractions of  Ethylalcohol Extracts of Fresh Parsley Roots and Its Allelopathic Effects on  Fusarium oxysporum f.sp.cucumberinum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年09
Page:
93-100
Research Field:
Publishing date:

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Title:
Separation of Allelochemicals of the Sixth Chromatography Fractions of  Ethylalcohol Extracts of Fresh Parsley Roots and Its Allelopathic Effects on  Fusarium oxysporum f.sp.cucumberinum
Author(s):
SUN Honghuan1; WANG Yong2; LI Lei3; YUN Xingfu1
(1.College of Agronomy，Inner Mongolia Agricultural University，Hohhot,Inner Mongolia 010019;2.Research Institute of Vegetables,Inner Mongolia Academy of Agriculture & Animal Husbandry Sciences，Hohhot,Inner Mongolia 010010;3.College of Manzhouli,Inner Mongolia University，Manzhouli，Inner Mongolia 012400)
Keywords:
parsley fresh root; the sixth chromatography fractions of ethylalcohol extracts; chromatography and separation; Fusarium oxyporum f.sp.cucumerinum; allelopathy; allelochemicals
PACS:
-
DOI:
10.11937/bfyy.201709020
Abstract:
Athylalcohol extracts of fresh parsley roots was used as the experimental material,and the methods has been applied with chromatography by a column with silicone coating,allelopaphy testing and GC-MS.We studied that ethylalcohol extracts of fresh parsley roots has been proceed with the fifth and sixth chromatography fractions and incorporated the different degree column fractions into PDA medium and co-cultured the plates with F.oxysporum f.sp. cucumberinum.The best fractions of ethylalcohol extracts of parsley fresh root were screened according to the allelopathic inhibition effect.At last，further allelochemicals of the sixth chromatography was identified by GC-MS.So as to achieve the purpose of identifing inhibitory allelochemicals released into the soil by parsley crop.The results showed that there were significant different allelopathic effect for the degree column fractions obtained from the fifth and sixth cycles of chromatography respectively on F.oxysporum f.sp. cucumberinum.The allelopathic effects of the fifth optimal fractions (RE211018,RE29554,RE62359 and RE68656) compared with the fifth cycle tomographic ethylalcohol (the control，ECK1) was 63.05%,70.84%,69.38% and 59.16% respectively.The allelopathic effects of the sixth optimal fractions (RE2110186,RE295548,RE623597 and RE686568) compared with the sixth cycle tomographic ethylalcohol (the control，ECK2) was 70.14%,71.38%,64.84% and 60.63% respectively.There were 11 kinds of allelochemicals contained in Phenol，ester，amine and alkanes 4 classes in the GC-MS analysis of the sixth chromatography fractions of ethylalcohol extracts of parsley fresh root.They were 2,4-bis (1,1-dimethylethyl)-phenol，8,11-octadecadienoic acid,methyl ester，Ethyl 13-docosenoate (ethyl erucate)，Dimethyl phthalate,1,2-benzenedicarboxylic acid，butyl 2-methylpropyl ester，1,2-benzenedicarboxylic acid,butyl 2-ethylhexyl ester,phthalic acid，butyl 2-pentyl ester,dibutyl phthalate,9-Octadecenamide,(Z)-,heptacosane，3-ethyl-5-(2-ethylbutyl)-octadecane.

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Last Update: 2017-05-15