|Table of Contents|

‘Gala’ Apple Leaf Callus Induction(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年09
Page:
88-92
Research Field:
Publishing date:

Info

Title:
‘Gala’ Apple Leaf Callus Induction
Author(s):
GAO Bing12SUN Jun13
(1.College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095;2.Department of Horticulture,Shanxi Forestry Vocational and Technical College,Taiyuan,Shanxi 030009;3.College of Horticulture,Anhui Agricultural University,Hefei,Anhui 230036)
Keywords:
appleleaf bladecallusgrowth regulatordarknesstransgenerational cycle
PACS:
-
DOI:
10.11937/bfyy.201709019
Abstract:
Apple as the ‘Gala’ single bud somaclone leaf as explant callus induction,callus induction were studied in the process of plant growth regulators types and concentration ratio,light and dark conditions and transgenerational days on its formation and growth.The results showed that the combination could be more efficiently induce the formation of callus,MS + sugar 30 g?L-1+ 2,4-D 2.0 mg?L-1+ NAA 0.5 mg?L-1+ BA 2.0 mg?L-1 induced efficient;the formation of callus needed 14-21 days of dark raise,transgenerational cycle with 15-30 days was advisable.

References:

 

[1]高海秀.苹果原生质体培养研究[J].现代农业科学,2016(13):105,108.

[2]赵恺,刘伟,刘忠巍,.苹果组织培养研究概况[J].河北果树,2011(5):1-3.

[3]崔美,焦齐庆,陈学森,.苹果叶片愈伤组织的诱导培养[J].山东农业科学,2012,44(3):17-20.

[4]于丽艳,王志和,周波,.苹果离体叶片再生体系两步培养法的研究[J].落叶果树,2005(2):5-8.

[5]韩焱,王光林,邢卓,.观赏性苹果“红霞”的组织培养和快速繁殖[J].植物生理学通讯,2005,41(2):188.

[6]潘增光,邓秀新.苹果原生质体分离培养及植株再生[J].园艺学报,2000,27(2):95-101.

[7]魏国芹,李鼎立,梁美霞,.苹果原生质体培养再生愈伤组织[J].中国农学通报,2009,25(20):179-186.

[8]冯晓明.苹果bHLH基因MdcmHLHl克隆及功能分析[D].泰安:山东农业大学,2011.

[9]柴丽娟.苹果叶片再生体系的建立与优化[D].兰州:甘肃农业大学,2007.

[10]焦其庆,陈学森,郑巧娜,.苹果周期蛋白依赖性蛋白激酶亚基基因MdCKS表达载体的构建[J].山东农业科学,2011(8):1-3.

[11]崔凯荣,戴若兰.植物体细胞胚发生的分子生物学[M].北京:科学出版社,2000:329-397.

[12]范双喜.芦笋花药培养中影响因素的研究[J].北京农业科学,1993,11(6):23-25.

[13]王震星,张磊.枣花药培养再生植株及其染色体倍性研究[J].北方果树,1998(2):5-6.

[14]曹孜义,李唯,张利平.三倍体葡萄花药植株的诱导[J].科学通报,1993,38(5):451-454.

[15]季华译.杨树单倍体的生产[J].国外林业,1996,26(2):14-17.

[16]韩晓红,李磊,段春红.不同激素对红豆杉愈伤组织诱导及增殖的影响[J].绿色科技,2013(1):173-174,178.

[17]张文泉,闫伟.红花槐愈伤组织诱导及植株再生的研究[J].内蒙古农业大学学报,2013,34(1):34-41.

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Last Update: 2017-05-15