|Table of Contents|

Establishment of Regeneration System for Cucumis melo L.cv.‘Huangdanzi’ of Melon(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年09
Page:
85-88
Research Field:
Publishing date:

Info

Title:
Establishment of Regeneration System for Cucumis melo L.cv.‘Huangdanzi’ of Melon
Author(s):
TIAN Fang; YAO Zhaoqun; CHEN Meixiu; XU Ying; ZHAO Sifeng
(College of Agriculture,Shihezi University/Key Laboratory at the Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization,Shihezi,Xinjiang 832003)
Keywords:
melon; ‘Huangdanzi’; tissue culture; adventitious buds; regeneration system
PACS:
-
DOI:
10.11937/bfyy.201709018
Abstract:
Cucumis melon L．cv.‘Huangdanzi’ cotyledon were used as test materials,using the method of tissue culture,effects of the cotyledon day age,different concentrations of 6-BA,IAA and cefotaxime on adventitious buds were studies.The results showed that the cotyledon age played an important role in inducing adventitious buds,the optimal induction time was two days after cotyledon cultured under light,the regeneration rate was 80%.In addition,MS+6-BA 1.2 mg?L-1 was the best induction medium,the regeneration rate was 67%.Furthermore,500 mg?L-1 cefotaxime was the best concentration that could be used to eradicate agrobacterium，25 mg?L-1 kanamycin was the best screening concentration.

References:

 

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Last Update: 2017-05-15