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Effects of Purified Humic Acid on Cucumber Germination and Physiological and  Biochemical Characteristics Under NO3- Stress(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年09
Page:
1-8
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Title:
Effects of Purified Humic Acid on Cucumber Germination and Physiological and  Biochemical Characteristics Under NO3- Stress
Author(s):
GU Duanyin1GAO Junjie1JIAO Juan1WANG Xiufeng23
(1.Taian Academy of Agricultural Sciences,Tai′an,Shandong 271000;2.College of Horticulture Science and Engineering,Shandong Agricultural University,Tai′an,Shandong 271018;3.State Key Laboratory of Crop Biology,Tai′an,Shandong 271018)
Keywords:
PHANO3- stresscucumberseed germinationantioxidant enzyme activities
PACS:
-
DOI:
10.11937/bfyy.201709001
Abstract:
In order to explore the effects of PHA on cucumber seed germination and physiological and biochemical characteristics under NO3- (75 mmol?L-1) stress,‘Xintai Mici’ and ‘Jinyou No.1’ cucumber seeds were used as experimental cultivars,treated with different PHA concentration (0,25,50,100,150 mg?L-1).The results showed that seed germination,radicle length,lateral root length,lateral root number,hypocotyl length and diameter,and seedling fresh weight were decreased under NO3- stress.The above indices of adding PHA treatments were increased obviously and showed that PHA could partly alleviate NO3- stress.The seedling electrolyte leakage rate and seed cotyledon α-amylase activity were decreased but radicle dehydrogenase activity was increased under nitrate stress.MDA and soluble protein content,SOD,POD,CAT activity were increased.After adding PHA,the seedling electrolyte leakage rate was significantly reduced,but with the increasing concentration of PHA the decreases difference became smaller.Cotyledon α-amylase activity and radicle dehydrogenase activity were increased and showed downward trend when added high PHA concentration.Radicle and hypocotyl MDA content decreased when treated with PHA but increased with high PHA concentration.Soluble protein content were increased but decreased with high PHA concentration.SOD,POD,CAT activity showed a downward trend treated with PHA compared with NO3- stress.Cucumber seed under NO3- stress was alleviated by adding PHA so that cucumber seedling growth became normal.The physiological and biochemical indices during cucumber seeds germination under NO3-stress and PHA treatments showed difference in different varieties and different organs100 mg?L-1 PHA treatment showed the best effects.

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Last Update: 2017-05-11