Tissue Culture and Regeneration of Platycodon grandiflorus in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: LIU XiuJie1; 2; TIAN Ludi2; PEI Yi2; WANG Ji2; LIU Yanjun2; NIE Jiangli2; 1.Tianjin Cuiping Lake Science Park,Tianjin 301908;2.College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300384

Keywords:: Platycodon grandiflorus; culture medium; regeneration; sterile seedling

Abstract:: Cotyledons and hypocotyls of Platycodon grandiflorus were used as explants to analyze the bud induction frequencies of them by in vitro culture method,so as to find out the ideal plants types and the corresponding culture conditions.In order to establish an efficient regeneration system of tissue culture of Platycodon grandiflorus and provide an effective method for the breeding and utilization of fine strains.The results showed that both the cotyledons and hypocotyls of Platycodon grandiflorus regenerated buds successfully.Cotyledons′ induction rate was the highest，the most suitable medium for the MS+ 1.0 mg·L^-1 BA+0.5 mg·L^-1 IAA，the highest growth of Platycodon grandiflorus regeneration bud length in the medium of MS+0.5 mg·L^-1BA+1.0 mg·L^-1 IAA.Thus,the cotyledon explants of Platycodon grandiflorus was the most ideal explant type,the corresponding differentiation medium was MS+ 1.0 mg·L^-1 BA+0.5 mg·L^-1 IAA,elongation medium combination MS+0.50 mg·L^-1 BA+1.0 mg·L^-1 IAA.

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Last Update: 2017-09-29