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Tissue Culture and Regeneration of Platycodon grandiflorus in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年17
Page:
40-43
Research Field:
Publishing date:

Info

Title:
Tissue Culture and Regeneration of Platycodon grandiflorus in vitro
Author(s):
LIU XiuJie12TIAN Ludi2PEI Yi2WANG Ji2LIU Yanjun2NIE Jiangli2
1.Tianjin Cuiping Lake Science Park,Tianjin 301908;2.College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300384
Keywords:
Platycodon grandiflorusculture mediumregenerationsterile seedling
PACS:
-
DOI:
10.11937/bfyy.20170888
Abstract:
Cotyledons and hypocotyls of Platycodon grandiflorus were used as explants to analyze the bud induction frequencies of them by in vitro culture method,so as to find out the ideal plants types and the corresponding culture conditions.In order to establish an efficient regeneration system of tissue culture of Platycodon grandiflorus and provide an effective method for the breeding and utilization of fine strains.The results showed that both the cotyledons and hypocotyls of Platycodon grandiflorus regenerated buds successfully.Cotyledons′ induction rate was the highest,the most suitable medium for the MS+ 1.0 mg·L-1 BA+0.5 mg·L-1 IAA,the highest growth of Platycodon grandiflorus regeneration bud length in the medium of MS+0.5 mg·L-1BA+1.0 mg·L-1 IAA.Thus,the cotyledon explants of Platycodon grandiflorus was the most ideal explant type,the corresponding differentiation medium was MS+ 1.0 mg·L-1 BA+0.5 mg·L-1 IAA,elongation medium combination MS+0.50 mg·L-1 BA+1.0 mg·L-1 IAA.

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Last Update: 2017-09-29