|Table of Contents|

Tissue Culture and Rapid Propagation of Kaempferia galangal L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年19
Page:
11-15
Research Field:
Publishing date:

Info

Title:
Tissue Culture and Rapid Propagation of Kaempferia galangal L.
Author(s):
LIANG Chunhui1; HUANG Min1; LI Xiuping1; DONG Bin1; YAN Xiaodong2
1.Department of Tropical Crops,Guangdong Agriculture Industry Business Polytechnic College,Guangzhou,Guangdong 510507；2.Guangzhou Entry-Exit Inspectional and Quarantine Bureau,Guangzhou,Guangdong 510623
Keywords:
Kaempferia galangal L.; adventitious bud; tissue culture
PACS:
-
DOI:
10.11937/bfyy.20170859
Abstract:
The bud of Kaempferia galangal were was used as the materials,to study the effect of different factors on the disinfection,multiplication,root induction,so as to establish in vitro culture system and provide reference for detoxification.The results showed that the best disinfection method of the bulb explants of Kaempferia galangal was 75% ethanol for 60 seconds,followed by 0.1% HgCl2 8 minutes,the contamination rate was 13.33%,and the survival rate was 86.67%.The effects of hormones on proliferation of adventitious bud were diffferent,the appropriate medium for proliferation was MS+6-BA 2.00 mg?L-1+NAA 0.25 mg?L-1 with the multiplication coefficient of 6.30.The best medium for root induction was 1/2MS+NAA 0.50 mg?L-1 and the number of roots per plantlets was 15.32．

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Last Update: 2017-10-11