Identification and Physiological Characteristics of Pathogeny Caused Rusty Spot  Disease for Pleurotus ostreatus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Identification and Physiological Characteristics of Pathogeny Caused Rusty Spot  Disease for Pleurotus ostreatus

Author(s):: CHEN Xuefeng; WU Xiaojian; WANG Canqin; WU Shengjin; WEI Shiyan; (Institute of Microbiology，Guangxi Academy of Agricultural Sciences，Nanning，Guangxi 530007)

Keywords:: Pleurotus ostreatus; rusty spot disease; identification; physiological characteristics

Abstract:: Pleurotus ostreatus sporocarp naturally infected rusty spot disease that collected from Nanning，Guangxi was used as test material.The pathogen was isolated by tissue isolation and pathogenicity was tested following Koch′s postulate;identification of the pathogen was conducted by ITS 16S rDNA molecular identification.The suitable growth temperature，pH levels and salinity were detected by testing the bacteria biomass.Sensitivity of antibiotics of the pathogen was detected by Haloes methods.The results showed that the pathogen infected the Pleurotus ostreatus was Pseudomonas tolaasii，and the optimal conditions for its growth were as follows,temperature 21-30 ℃;pH 7-8;salinity 0.5%-2.5%.Streptomycin，tetracycline，gentamicin andterramycin could inhibit growth of pathogen and the MIC was 312.0,156.0,78.0，78.0 μg?mL-1，respectively.The pathogen was not sensitive to erythromycin and ampicillin.

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Last Update: 2017-05-05