|Table of Contents|

Optimization of Hairy Root Induction by Agrobacterium rhizogene in  Physalis pubescens L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年08
Page:
115-119
Research Field:
Publishing date:

Info

Title:
Optimization of Hairy Root Induction by Agrobacterium rhizogene in  Physalis pubescens L.
Author(s):
REN Ruyi; LI Sixuan; JIN Qiu; LIANG Shiru; WANG Shanshan
(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157011)
Keywords:
Physalis pubescens L.; Agrobacterium rhizogenes; hairy root; induction rate
PACS:
-
DOI:
10.11937/bfyy.201708026
Abstract:
Agrobacterium rhizogenes strains of A4,C58C1,R1601,ATCC15834 were used to infect the explants of Physalis pubescens L.,then the influences of different Agrobactrium rhizogenes species,explants types,different preculture times,different infection times and co-culture times and acetosyringone on hairy root induction rate were researched.Using PCR technique to identify the induced hairy roots.The results showed that Agrobacterium rhizogenes strains of A4,C58C1,R1601,ATCC15834 all could induce hairy roots,but inductivity of ATCC15834 was higher.The inductivity of leaf was higher than stem.The best induction conditions of hairy roots were preculturing for 2—3 days,infecting for 6-8 minutes,co-culturing for 2-3 days.Acetosyringone 100 μmol?L-1 would be necessary to upgrade transformation frequencies.PCR detection results indicated that the rolB gene in Ri plasmid of A.rhizogenes had integrated into the genome of P.pubescens and been expressed.

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Last Update: 2017-05-05