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Cloning and Expression Analysis of DAD-1 Gene in Osmanthus fragrans(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年08
Page:
96-102
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of DAD-1 Gene in Osmanthus fragrans
Author(s):
ZOU Jingjing1; LU Ling2; ZENG Xiangling1; WANG Caiyun3
(1.School of Nuclear Technology and Chemistry & Biology，Hubei University of Science and Technology，Xianning，Hubei 437100;2.Institute of Vegetables,Xiaogan Academy of Agricultural Sciences，Xiaogan,Hubei 432000;3.Key Laboratory for Biology of Horticultural Plants，Ministry of Education，Huazhong Agricultural University,Wuhan,Hubei 430070)
Keywords:
Osmanthus fragrans; DAD-1 gene; program cell death; flower senescence
PACS:
-
DOI:
10.11937/bfyy.201708022
Abstract:
Osmanthus fragrans ‘Liuye Jingui’ was used as tested material.The complete coding sequence (CDS) of DAD-1 gene was cloned by the method of thermal asymmetric interlaced PCR (TAIL-PCR)，and its expression patterns in different organs and different flowering stages in O.fragrans were analyzed，to provide reference for studying the function of this gene during the flower senescence.The results showed that the length of DAD-1 gene was constituted of 560 bases with an open reading frame of 351 bp and a 3′-Poly A.The DAD-1 protein encoded 116 amino acids，and it was a hydrophobic integration membrane protein with three alpha-helixs in the structure.The results of semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) showed that DAD-1 expressed in most tissues of sweet Osmanthus，such as the stem，root，flower and leaf，and it was higher in the young organs such as young flowers and new leaves，but lower in old organs such as the old leaves.The results of quantitative real-time polymerase chain reaction (real-time PCR) showed that DAD-1 abundance rapidly decreased at the initial flowering stage，much earlier than the visible senescence symptoms.

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Last Update: 2017-05-05