|Table of Contents|

Preliminary Screening of Lentinula edodes Mutant Strains by 60Co-γ Irradiation(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年15
Page:
151-157
Research Field:
Publishing date:

Info

Title:
Preliminary Screening of Lentinula edodes Mutant Strains by 60Co-γ Irradiation
Author(s):
WANG Fei; SONG Bing; LI Dan; FU Yongping; MENG Lingsi; LI Yu
(Engineering Research Center of Chinese Ministry of Edible and Medicinal Fungi,Jilin Agricultural University,Changchun,Jilin 130118)
Keywords:
Lentinula edodes; 60Co-γ; radiation mutagenesis; cellulose; hemicellulose
PACS:
-
DOI:
10.11937/bfyy.20170775
Abstract:
The Lentinus edodes strain ‘Qing 20’ was irradiated by 60Co-γ ray,and the mutagen dosage was respectively 0.4 kGy,0.6 kGy,0.8 kGy,dose rate was 10 Gy?min-1.Non-radiating treatment was CK.The effect of different mutagen dosages on the lethality rate of Lentinus edodes was studied.The mutant strains were identified by antagonistic reaction and SSR molecular marker,and utilization ratio of cellulose and hemicellulose was preliminarily screened.The results showed that all strains were alive after treated with 0.4 kGy,the death rate was 46.7% after treated with 0.6 kGy,and the death rate was 100% after treated with 0.8 kGy.Six mutant strains were screened out by antagonistic reaction and SSR,and the utilization ratio of cellulose and hemicellulose were better than CK.

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Last Update: 2017-08-04