|Table of Contents|

Antibody Titer Detection and Expression Characteristic Analysis in Fruit of Type II Tomato Metacaspase SlMC7 Protein(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年16
Page:
50-55
Research Field:
Publishing date:

Info

Title:
Antibody Titer Detection and Expression Characteristic Analysis in Fruit of Type II Tomato Metacaspase SlMC7 Protein
Author(s):
WANG Zhichao1; 2; MA Qiumin2; YE Yanxiong2; WEN Shuai2; CAO Jingshun2; QU Guiqin2
1.Henan Provincial Food and Drug Evaluation and Inspection Center，Zhengzhou，Henan 450046；2.College of Food Science and Nutritional Engineering，China Agricultural University，Beijing 100083
Keywords:
metacaspase protein; antibody; protein expression; tomato
PACS:
-
DOI:
10.11937/bfyy.20170730
Abstract:
SlMC7 prokaryotic expression recombinant protein was used as test materials.SlMC7 polyclonal antiserum was obtained from immunized mice by using purified SlMC7 recombinant protein from Escherichia coli.ELISA and protein dot plot methods were used to detect the antibody titer and expression characteristics and the expression difference at different stages，providing material foundation and reference basis for subsequent SlMC7 functional research in tomato fruits.The results showed that the titer of SlMC7 polyclonal antibodies was more than 1∶256 000 and sensitivity of SlMC7 polyclonal antibodies was 1.6 ng at the dilution of 1∶10 000.SlMC7 protein was detected at different stages，SlMC7 had a higher expression in breaker fruits.SlMC7 might be relevant to the fruit ripening of tomato.

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Last Update: 2017-08-29