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¡¶±±·½Ô°ÒÕ¡·[ISSN:1001-0009/CN:23-1247/S]

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2017Äê07

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105-115

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Title:

Molecular Identification Analysis of Six Newª« S-RNase ª«Genes Controlling ª¤ Self-incompatibility From Xinjiang Wild Almond£¨ª«Prunus tenellaª« Batsch.£©

Author(s):

ZENG Bin1; 2; LIU Mengwen1; WANG Jianyou3; WANG Bo1ª¤

(1.College of Forestry and Horticulture£¬Xinjiang Agricultural University£¬Urumqi£¬Xinjiang 830000£»2.Research Center for Xinjiang Characteristic Fruit Tree£¬Xinjiang Agricultural University£¬Urumqi£¬Xinjiang 830000£»3.Branch of Xinjiang,China Academy of Forestry Sciences£¬Urumqi£¬Xinjiang 830000)ª¤

Keywords:

Prunus tenellaª«; natural population; self-incompatibility; ª«S-RNase ª«gene; clone and identification

PACS:

-

DOI:

10.11937/bfyy.201707023

Abstract:

Takingª«Prunus tenellaª« Batsch.(wild almond) as materials,which is a very primitive tree species,precious,ancient and very scarce in the world today.The use of Rosaceae self-incompatibility primer combinations, using ª«S-RNaseª« allele PCR amplification,RT-PCR and RACE technology,the ª«S-RNaseª« gene of Xinjiang wild almond was studied,in order to further study for the Xinjiang wild almond molecular mechanism of self-incompatibility.The results showed that six new self-incompatibility ª«S-RNaseª« genes were successfully cloned from the pistils of the wild almond from five natural populations different individuals sample,which were named ª«PtS10ª« (GeneBank accession number£ºKJ755352),ª«PtS11ª« (GeneBank accession number£ºKJ755353),ª«PtS12ª« (GeneBank accession number£ºKJ755354),ª«PtS13ª« (GeneBank accession number£ºKJ755355),ª«PtS14ª« (GeneBank accession number£ºKJ755356) and ª«PtS15ª«(GeneBank accession number£ºKJ755357)£»and the six new genes were analyzed by bioinformatics study.These studies provided basic information for the further study of molecular mechanism of self-incompatibility in Xinjiang wild almond.ª¤

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