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Efficiency of Rapid Propagation of Different Grape Varieties(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年19
Page:
29-34
Research Field:
Publishing date:

Info

Title:
Efficiency of Rapid Propagation of Different Grape Varieties
Author(s):
QIAO Haiyu; XIANG Xiaodong; REN Genzeng; CUI Jianghui; CHANG Jinhua
College of Agriculture,Hebei Agricultural University,Baoding,Hebei 071001
Keywords:
grape; rapid regeneration; meristem culture
PACS:
-
DOI:
10.11937/bfyy.20170685
Abstract:
Three different ploidy grape tetraploid ‘Muscat Hamburg’‘Jumei’,diploid ‘Muscat Hamburg’ were used as materials in this study.The effect of different hormones and hormone concentration in the tissue culture and different sampling time on the efficiency of rapid propagation.The results showed that the optimum sampling time was in mid-May,the suitable medium for the induction and subculture were MS+6-BA 1.1 mg?L-1+KT 0.5 mg?L-1；MS+6-BA 1.1 mg?L-1+KT 0.5 mg?L-1.In the same culture condition,adventitious bud proliferation times of the tetraploid ‘Muscat Hamburg’ and tetraploid ‘Jumei’ was higher than diploid ‘Muscat Hamburg’.In the rooting process,diploid ‘Muscat Hamburg’ showed better than ‘Jumei’ and tetraploid ‘Muscat Hamburg’.The optimum rooting medium were 1/2MS+NAA 0.30 mg?L-1,1/2MS+IAA 0.50 mg?L-1 and 1/2MS+IBA 0.10 mg?L-1 respectively for three grape cultivars.The optimum mixed ratio of vermiculite and peat was 1∶1 for acclimatization,the survival rate was more than 90%.

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Last Update: 2017-10-11