|Table of Contents|

Photosynthetic Characteristics of Rhodiola sachalinensis Transformed by  UDP-glucose：flavonoid 3-O-glucosyltransferase Gene(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年06
Page:
108-112
Research Field:
Publishing date:

Info

Title:
Photosynthetic Characteristics of Rhodiola sachalinensis Transformed by  UDP-glucose：flavonoid 3-O-glucosyltransferase Gene
Author(s):
WEI Xiaowei; NING Siqi; YANG Bin; ZHANG Xin; XU Hongwei; ZHOU Xiaofu
(Key Laboratory for Plant Resources Science and Green Production,Jilin Normal University,Siping,Jilin 136000)
Keywords:
UDP-glucose：flavonoid 3-O-glucosyltransferase; Rhodiola sachalinensis; chlorophyll fluorescence
PACS:
-
DOI:
10.11937/bfyy.201706025
Abstract:
Rhodiola sachalinensis was used as test material.Modulated chlorophyll fluorescence system (IMAGING-PAM M-Series) was used to measure chlorophyll fluorescence parameters of Rhodiola sachalinensis transformed by UDP-glucose：flavonoid 3-O-glucosyltransferase gene to compare the chlorophyll fluorescence characteristics difference of Rhodiola sachalinensis and provided the scientific basis for germplasm improvement.The results showed that when exposed to photosynthetic active radiation (PAR) of 55 μmol?m-2?s-1,the FaGT1 gene transgenic Rhodiola sachalinensis,the FaGT1i gene transgenic Rhodiola sachalinensis and control had no significant difference in the value of maximum photosystem II quantum yield,the potential activity of photosystem II,non-photochemical quenching,photochemical quenching and effective quantum yield were significant higher in the FaGT1 gene transgenic Rhodiola sachalinensis than in the FaGT1i gene transgenic Rhodiola sachalinensis and control,but the regulated non-photochemical energy dissipation and non-regulated energy dissipation were lower.In addition,the differences of the electron transport rate,photochemical quenching and effective quantum yield in the FaGT1 gene transgenic Rhodiola sachalinensis,the FaGT1i gene transgenic Rhodiola sachalinensis and control under different PAR conditions.The electron transport rate and effective quantum yield were after the first increase in the stability of the trend and that significant higher in the FaGT1 gene transgenic Rhodiola sachalinensis than the FaGT1i gene transgenic Rhodiola sachalinensis and control,but the photochemical quenching was after the first decline in a smooth trend and had no significant difference.The primary light energy conversion of photosystem Ⅱ and the potential photosynthetic activity had increased of the FaGT1 gene transgenic Rhodiola sachalinensis. The energy metabolism of plant itself has been improved.The results above provided scientific basis for the cultivation of Rhodiola sachalinensis germplasm．

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Last Update: 2017-04-05