|Table of Contents|

Establishment of ISSR Reaction System and Genetic Diversity Analysis of Hypericum attenuatum Choisy.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年05
Page:
96-101
Research Field:
Publishing date:

Info

Title:
Establishment of ISSR Reaction System and Genetic Diversity Analysis of Hypericum attenuatum Choisy.
Author(s):
WANG Xia1; 2; XU Jize1; 2; WANG Huan1; 2; GAO Qianqian1
（1.College of Biological Engineering,College of Jilin Agriculture Science and Technology，Jilin，Jilin 132101;2.Key Laboratory of Changbai Mountain Animal and Plant Rescources′ Utilization and Protection of Universities in Jilin Province，Jilin,Jilin 132101)
Keywords:
Hypericum attenuatum; ISSR reaction system; genetic diversity
PACS:
-
DOI:
10.11937/bfyy.201705023
Abstract:
Fourteen Hypericum attenuatum were used as materials,the ISSR-PCR reaction system was established and optimized by L16(45) orthogonal experimental design.The genetic diversity of Hypericum attenuatum was analyzed by the optimal reaction system.The results showed that the optimum reaction system contained 1.0 U Taq DNA polymerase，0.2 mmol?L-1 dNTs，2.0 mmol?L-1 Mg2+ and 45 ng DNA template respectively in a 20 μL ISSR reaction system.Eleven polymorphic primers which were used to analyze the genetic diversity of Hypericum attenuatum were selected from 45 ISSR primers.A total of 85 bands were amplified，of which 57 bands were polymorphic loci and the percentage of total polymorphic loci was 67.06%.The genetic similarity coefficient was 0.375 1-0.725 2 in 4 populations of Hypericum attenuatum,and the average was 0.541 6.The clustering analysis was constructed by UPGMA method based on the genetic identity and 14 Hypericum attenuatum materials were clustered into 3 main groups and 4 subgroups.The level of genetic diversity of Hypericum attenuatum between populations was higher than that within populations and between the genetic distance and geographic distance had a certain correlation.

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Last Update: 2017-03-24