|Table of Contents|

Isolation,Identification and Training for a Wild Fungus of Genus Leucopaxillus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年18
Page:
161-166
Research Field:
Publishing date:

Info

Title:
Isolation,Identification and Training for a Wild Fungus of Genus Leucopaxillus
Author(s):
LIU Xiaoting1; SUN Guoqin2; LI Yajiao2; WANG Shuyan1; GUO Jiufeng1
1.Key Laboratory of Ion Beam Biotechnology/College of Physical Science and Technology,Inner Mongolia University,Huhhot,Inner Mongolia 010021;2.Inner Mongolia Academy of Agriculture and Livestock Sciences,Hohhot,Inner Mongolia 010031
Keywords:
wild fungi; morphological characteristics; ITS gene sequence; molecular identification methods; primordium
PACS:
-
DOI:
10.11937/bfyy.20170461
Abstract:
A wild mushroom from Inner Mongolia′s Xilingol grassland was identified by methods of morphological characteristics and ecological habits of identification and molecular identification.Genomic DNA was extracted from mycelium then used as template for the 18S and ITS gene PCR amplification and sequencing.The 18S and ITS gene sequences were submitted to NCBI and aligned by Blast.The results showed that the fungi belonged to the genus Leucopaxillus.In addition,it had been found experimentally that the mycelium could be formed primordium in MS plate culture medium in dark.The ITS sequence was uploaded into NCBI,and GenBank accession number was KY173356.

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Last Update: 2017-10-07