In vitro Culture and Plant Regeneration of Ardisia gigantifolia Stapf.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: In vitro Culture and Plant Regeneration of Ardisia gigantifolia Stapf.

Author(s):: FU Yunliu1; 2; XU Li1; 2; LI Zhiying1; 2; HUANG Bilan1; 2; LI Kelie1; 2; (1.Institute of Tropical Crop Genetic Resources，Chinese Academy of Tropical Agricultural Sciences，Danzhou，Hainan 571737；2.Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation，Danzhou，Hainan 571737)

Keywords:: Ardisia gigantifolia Stapf.; leaf; in vitro culture; regenerated plantlet

Abstract:: A.gigantifolia Stapf.leaves were used as explants，the effects of different mediums on leaf callus induction，adventitious bud differentiation，proliferation and rooting were studied.The results showed that the callus induced from leaf explants on medium MS+6-BA 1.0 mg?L-1+NAA 1.0 mg?L-1 were easy to produce auxiliary shoots.The differentiation rate was 88.9%.The suitable medium for bud subculture and proliferation was MS+6-BA 2.0 mg?L-1+NAA 0.1 mg?L-1.The elongation medium was MS+6-BA 0.5 mg?L-1+NAA 0.1 mg?L-1+10% coconut water.The optimal rooting medium was MS+NAA 0.1 mg?L-1+IBA 1.0 mg?L-1 with rooting rate as high as 100%.The roots developed well and the plantlets grew vigorously.The plantlets with well-developed roots were successfully acclimatized and planted with a survival rate of 85%.

[1]中国科学院中国植物志编写委员会.中国植物志:第58卷[M].北京:科学出版社,1979:93.

[2]江苏新医学院.中药大辞典：上册[M].上海:上海人民出版社,1997:1087.

[3]广西壮族自治区卫生厅.广西中药材标准[M].9版.南宁:广西科学技术出版社,1992:58.

[4]唐亚平.中药走马胎治疗类风湿关节炎的临床观察[J].四川中医,2007,25(1):54-55.

[5]唐文秀,骆文华,隗红燕,等.萘乙酸对野生药用植物走马胎扦插繁殖的影响[J].江苏农业科学,2010(4):241-243.

[6]黄美娟,刘小辉,邓娅玲，等.朱砂根的组织培养和快速繁殖[J].植物生理学通讯,2007,43(6)：1149-1150.

[7]罗吉凤,程治英,龙春林.虎舌红的组织培养[J].植物生理学通讯,2004,40(4):465-466.

[8]GOO D H，KWON O K，LEE Y R，et al.Micropropagation of Ardisia pusilla and Ardisia japonica in vitro[J].Acta Hort(ISHS),2008,766:237-242.

[9]王刘圣丹，邱丝丝，夏国华，等.堇叶紫金牛的组织培养与快速繁殖[J].植物生理学通讯，2010，46（6）：615-616.

Last Update: 2017-03-23