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Identification and Sequence Analysis of Shouguang Isolate by RT-PCR of Pepper Mild Mottle Mosaic Virus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年04
Page:
94-97
Research Field:
Publishing date:

Info

Title:
Identification and Sequence Analysis of Shouguang Isolate by RT-PCR of Pepper Mild Mottle Mosaic Virus
Author(s):
TANG Yuhai1; QIAO Ning2; SUN Xiaohui2
(1.Jiasixie Agricultural College，Weifang University of Science and Technology，Shouguang，Shandong 262700；2.College of Plant Protection，Shandong Agricultural University/Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests，Tai′an，Shandong 271018)
Keywords:
pepper mild mottle mosaic virus; Shouguang isolate; RT-PCR; sequence analysis
PACS:
-
DOI:
10.11937/bfyy.201704021
Abstract:
Peppers infected by PMMoV in Shouguang city,Shandong Province were used as test material and kit was used to extract total RNA from infected pepper and total RNA was further reversed into cDNA by reverse transcription.PMMoV-F and PMMoV-R was synthesized according to reported specific primers.The cDNA of PMMoV isolate was used for PCR amplication.The results showed that the molecular weight of band was 576 bp.Sequence analysis showed that PMMoV infected pepper in Shouguang had closer genetic relationship with PMMoV isolated in other areas，which could achieve 99%-100%.

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Last Update: 2017-03-23