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Molecular Identification and Phylogenetic Analysis of Two Wild Edible Mushrooms in Chengde Area(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年17
Page:
172-177
Research Field:
Publishing date:

Info

Title:
Molecular Identification and Phylogenetic Analysis of Two Wild Edible Mushrooms in Chengde Area
Author(s):
ZHANG Jian1; FU Yajuan2
1.Langfang Polytechnic Institute,Langfang,Heibei 065000；2.College of Life Science,Langfang Normal University/Edible and Medicinal Fungi Research and Development Center of Hebei Universities/Langfang Key Laboratory of Edible Fungi Technology,Langfang,Heibei 065000
Keywords:
red mushroom; white mushroom; ITS; molecular identification; phylogenetic analysis
PACS:
-
DOI:
10.11937/bfyy.20170330
Abstract:
In order to elucidate taxonomic status and phylogenetic relationship of wild red mushroom and white mushroom in Chengde area,the ITS regions of rDNA genes were amplified using ITS1 and ITS4 as primers,cloned and sequenced；ITS sequences were then analyzed via NCBI blast program,meanwhile phylogenetic tree were constructed using MEGA 5.0 software,respectively.The results indicated that the similarity of between red mushroom from Chengde area and Chroogomphus rutilus（Accession No.：KR082876）was up to 99%,and genetic distance was 0.002.White mushroom, which was another kind of precious wild edible fungi in Chengde area,also had 99% similarity with Hygrophorus queletii (Accession No.：JF908069),and their genetic distance reached to 0.002.Therefore,red mushroom and white mushroom in Chengde area were at the molecular level identified as C.rutilus and H.queletii,respectively.The elucidation of their taxonomic status would be beneficial for identification,biological characteristics,and development and utilization of wild red mushroom and white mushroom.

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Last Update: 2017-09-30