|Table of Contents|

Effect of Chilling Stress on Expression Characteristic of Oxidosqualene Cyclase (OSC) in Callus of Panax ginseng(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年17
Page:
164-168
Research Field:
Publishing date:

Info

Title:
Effect of Chilling Stress on Expression Characteristic of Oxidosqualene Cyclase (OSC) in Callus of Panax ginseng
Author(s):
LIU Jia1; 2; FU Shuang1; WANG Meng1; WANG Jingran1; QUAN Xueli1; WU Songquan1; 2
1.Agricultural College,Yanbian University，Yanji，Jilin 133002；2.Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules of Ministry of Education，Yanbian University，Yanji，Jilin 133002
Keywords:
Panax ginseng; callus; chilling stress; oxidosqualene cyclase; expression characteristic
PACS:
-
DOI:
10.11937/bfyy.20170322
Abstract:
The continuous (5 ℃) and intermittent (25 ℃/5 ℃,12 h/12 h) chilling treatments were performed by three weeks fresh callus，which were harvested after 0，1，2，3 and 4 days treatment and recorded as CK，D1，D2，D3 and D4，respectively，the expressions of five kinds of oxidosqualene cyclase (OSC) genes in different treatments were detected by real-time quantitative PCR.The results showed that the expression of PgDAS was significantly induced in the D4 of continuous chilling treatment and D3 of intermittent chilling treatment，which were 2.15 times and 2.12 times of the control group，respectively；whereas Pgβ-AS1 and Pgβ-AS2expression were only induced in the D2 of continuous chilling stress treatment，which were 1.27 times and 1.71 times of the control group，respectively；PgCAS and PgLOS expression were not induced in both two kinds of chilling stress treatments.It indicated that the PgDAS maight play a key role in response to different chilling stress treatments，and the other OSC genes might be synergistic expression with PgDAS.

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Last Update: 2017-09-29