Effect of Different Cultivation Soil on Content of Dendrobium huoshanense Total Polysaccharide(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Effect of Different Cultivation Soil on Content of Dendrobium huoshanense Total Polysaccharide

Author(s):: YAO Liang1; 2; YU Nianjun1; 2; PENG Daiyin1; 2; CHEN Weidong2; WANG Xinyu1; 2; HE Xianglin3; (1.Anhui Academy of Chinese Medicine，Anhui University of Chinese Medicine,Hefei，Anhui 230012；2.Institute of Traditional Chinese Medicine Resources Protection and Development,Institute of Drug Metabolism，Hefei，Anhui 230012;3.Huoshan Changchong Chinese Medicine Development Co.Ltd.，Huoshan，Anhui 237266)

Abstract:: Taking Dendrobium huoshanense as test material，Dendrobium huoshanense were picked in harvest period which were cultivated in different cultivation soil and the content of total polysaccharide of which were determined by phenol-sulfuric acid method,to explore the effects of different cultivation soil on total polysaccharide content of Dendrobium huoshanense.The results showed that the content of total polysaccharide in different cultivation soil were：S1(sawdust+gravel，greenhouses)>S3(pine bark+gravel，greenhouses)>S4(coconut shell+gravel，greenhouses)>S2(gravel，greenhouses)>S5(gravel，bionic wild cultivation).There were significant differences among the total polysaccharide content Dendrobium huoshanense which cultivated in different cultivation soil as the results of statistical analysis.The cluster analysis data showed that Dendrobium huoshanense which cultivated in different cultivation substrate could be divided into four categories：S1 alone class；S3 a separate class；S4 a separate class；S2 and S5 a class.This study provided the reference for selecting the best type of cultivation soil of Dendrobium huoshanense and preliminary determining the type cultivation soil，in order to develope Dendrobium huoshanense resources adequately.

[1]包雪声,顺庆生,陈立钻,等.中国药用石斛[M].上海:复旦大学出版社,2001.

[2]肖强,杨丛,张峥,等.不同仿生态栽培方式对铁皮石斛多糖积累影响[J].中国农学通报，2015,31(10):142-147.

[3]姚亮,彭代银,俞年军,等.正交试验优化霍山米斛总多糖提取工艺[J].安徽中医药大学学报,2016,35(4):83-87.

[4]魏刚,顺庆生,李名海,等.中华仙草霍山石斛[M].成都:四川科学技术出版社,2015.

[5]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015.

[6]安彦峰,张雅琼,周路明,等.铁皮石斛栽培基质的研究进展[J].中国药房,2014,25(27):2581-2583.

[7]张毅.铁皮石斛的仿生栽培[D].南昌:南昌大学,2014.

[8]钱文林,张建霞,吴坤林,等.霍山石斛种苗繁殖与栽培研究[J].热带亚热带植物学报，2013,21(3):240-246.

Last Update: 2017-03-22