|Table of Contents|

Identification of Antagonistic Strain Against Cucumber Fusarium Wilt and Optimization of Its Fermentation Medium(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年03
Page:
132-137
Research Field:
Publishing date:

Info

Title:
Identification of Antagonistic Strain Against Cucumber Fusarium Wilt and Optimization of Its Fermentation Medium
Author(s):
DU Bin
(Department of Horticulture,Taiyuan College，Taiyuan,Shanxi 030012)
Keywords:
cucumber Fusarium wilt; Paenibacillus; response surface methodology; spore
PACS:
-
DOI:
10.11937/bfyy.201703030
Abstract:
Based on the morphological，physiological and 16S sRNA sequence characteristics，a bacterial strain was identified as Paenibacillus，named KC24.Bacteriostatic spectrum of KC24 was detected.The results showed that KC24 could confront a variety of plant pathogens，it had a broad-spectrum antimicrobial activity.The fermentation medium components for spore production of KC24 was optimized by responsesurface methodology.The optimized number of spores in the fermented liquid was 13.5×108 cfu?mL-1，increased more than 107.7%.The optimal fermentation conditions were corn flour 20 g，soybean meal 5 g，KNO3 2.0 g，KH2PO4 2.59 g，NaCl 1.0 g，(NH4)2SO4 2.04 g，FeSO4 0.009 1 g，MgSO4?7H2O 1 g，H2O 1 L，pH 7.0.

References:

 

[1]韦巧婕,郑新艳,邓开英,等.黄瓜枯萎病拮抗菌的筛选鉴定及其生物防效[J].南京农业大学学报,2013,36(1)：40-46.

[2]郭晋云,胡晓峰,李勇,等.黄瓜枯萎病对黄瓜光合和水分生理特性的影响[J].南京农业大学学报,2011,34(1)：79-80.

[3]OMAR I，O’NEILL T M，ROSSALL S.Biological control of Fusarium crown and root rot of tomato with antagonistic bacteria and integrated control when combined with the fungicide carbendazim[J].Plant Pathology,2006,55：92-99.

[4]CHUNG S，KONG H，BUYER J S，et al.Isolation and partial characterization of Bacillus subtilis ME488 for suppression of soilborne pathogens of cucumber and pepper[J].Appl Microbiol Biotechnol,2008,80：115-123.

[5]PERSSON L.Abiotic characteristics of soils suppressive to Aphanomyces root rot[J].Soil Biology and Biochemistry,2000,32：1141-1150.

[6]肖爱萍,游春平,梁关平,等.香蕉枯萎病拮抗菌的筛选及其作用机制研究[J].植物保护，2006，32(4):53-56.

[7]卢彩鸽,张殿朋,刘伟成,等.一株甘蓝枯萎病拮抗细菌的筛选尧鉴定及其抑菌活性测定[J].华北农学报，2014，29(1):195-202.

[8]王小慧,张国漪,李蕊,等.拮抗菌强化的生物有机肥对西瓜枯萎病的防治作用[J].植物营养与肥料学报,2013,19(1)：223-231.

[9]权春善,王军华,徐洪涛,等.一株抗真菌解淀粉芽孢杆菌的分离鉴定及其发酵条件的初步研究[J].微生物学报，2006,46(1):7-12.

[10]张荣胜,梁雪杰,刘永锋,等.解淀粉芽胞杆菌Lx-11 生物发酵工艺优化[J].中国生物防治学报，2013，29(2):254-262.

[11]TANYILDIZI M S，ELIBOL M，OZER D.Optimization of growth medium forthe production of α-amylase from Bacillus amyloliquefaciens using response surface methodology[J].Journal of Chemical Technology and Biotechnology，2006(4):618-622.

[12]沈萍,范秀荣,李广武.微生物学实验[M].北京：高等教育出版社,1999:69-82.

[13]权春善,王军华,徐洪涛,等.一株抗真菌解淀粉芽孢杆菌的分离鉴定及其发酵条件的初步研究[J].微生物学报，2006，46(1):7-12.

[14]WILSON K H,BLITCHINGTON R B,GREENE R C.Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction[J].Journal of Clinical Microbiology,1990,28(9)：1942-1946.

[15]程凯,江欢欢,沈标,等.棉花黄萎病拮抗菌的筛选及其生物防治效果[J].植物营养与肥料学报,2011,17(16)：166-173.

[16]江欢欢,程凯,杨兴明,等.辣椒青枯病拮抗菌的筛选及其生物防治效应[J].土壤学报，2010,47(6)：1225-1231.

[17]梅新兰,赵青云,谭石勇,等.辣椒疫病拮抗菌株筛选、鉴定及其防效[J].应用生态学报,2010,21(10)：2652-2658.

[18]张慧,杨兴明,冉炜，等.土传棉花黄萎病拮抗菌的筛选及其生物效应[J].土壤学报,2008,45(6)：1095-1101.

[19]孙燕霞,张瑞清,张伟,等.一株黄瓜枯萎病拮抗菌的筛选和鉴定[J].生物技术,2009,19(6)：22-25.

[20]陆继臣,迟乃玉,窦少华,等.响应面法优化灰霉病生防菌CNY-04 培养条件[J].微生物学通报,2013(40):1414-1422.

[21]CAI M H，ZHOU X S，SUN X Q，et al.Statistical optimization of medium composition for aspergiolide a production by marine-derived fungus Aspergillus glaucus[J].Journal of Industrial Microbiology and Biotechnology，2009，36(3):381-389.

[22]孙力军，陆兆新，孙德坤.Bacillus amyloliquefaciens ES-2液体发酵抗菌脂肽培养基及其主要影响因子筛选[J].食品工业科技，2008，29(5):60-63.

[23]梁昌聪,郭立佳,刘磊,等.响应面法优化解淀粉芽孢杆菌C101 发酵培养基[J].生物技术通报，2014(8)：169-174.

[24]李康乐，张萍，石彦鹏,等.利用响应面法优化泰乐菌素发酵培养基[J].中国兽药杂志，2014，48(1)：20-25.

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Last Update: 2017-03-22