|Table of Contents|

Optimization of SSR-PCR Reaction System for Armeniaca sibirica(L.)Lam.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年03
Page:
115-120
Research Field:
Publishing date:

Info

Title:
Optimization of SSR-PCR Reaction System for Armeniaca sibirica(L.)Lam.
Author(s):
ZHANG Haokai1; DONG Shengjun1; LIU Mingguo1; ZHONG Weiping2; LU Caiyun1; JIN Ling1
(1.College of Forestry，Shenyang Agricultural University，Shenyang，Liaoning 110866；2.College of Forestry,Nanjing Forestry University,Nanjing,Jiangsu 210037)
Keywords:
Armeniaca sibirica(L.)Lam.; SSR-PCR; reaction system; optimization
PACS:
-
DOI:
10.11937/bfyy.201703027
Abstract:
Four Armeniaca sibirica(L.)Lam groups were used as test materials，by orthogonal test L16(45)，the optimal SSR-PCR reaction system for Armeniaca sibirica(L.)Lam.was established.The results showed that the optimal systems of SSR（20 μL）was DNA templates 20 ng，primer 0.15 μmol?L-1,Mg2+ 2.0 mmol?L-1,Taq polymerase 1.0 U，dNTPs 0.25 mmol?L-1.After DNA amplification of 43 Armeniaca sibirica(L.)Lam.by using primer Y45，the products were 100-200 bp with good polymorphism and high stability，which could be used for Armeniaca sibirica(L.)Lam.SSR molecular marker development.

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Last Update: 2017-03-22