|Table of Contents|

SCoT-PCR Reaction System for Rubus biflorus Buch(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年03
Page:
109-111
Research Field:
Publishing date:

Info

Title:
SCoT-PCR Reaction System for Rubus biflorus Buch
Author(s):
YUAN Lei1; LIU Yu2; ZHONG Zhengchang2; XIE Bo2; CAO Dongxing2
(1.Office of Scientific Research Administration,Tibet Agriculture and Animal Husbandry College，Linzhi,Tibet 860000；2.College of Food Science，Tibet Agriculture and Animal Husbandry College，Linzhi,Tibet 860000)
Keywords:
Rubus biflorus Buch; SCoT; optimization
PACS:
-
DOI:
10.11937/bfyy.201703025
Abstract:
Rubus biflorus Buch was used as test material，total genomic DNA was extracted by using improved CTAB method.The factors including primers，dNTPs，Mg2+ and annealing temperature were studied to establish optimized SCoT-PCR reaction system to ensure the stability of SCoT for Rubus biflorus Buch.The results showed that the optimized reaction system of SCoT-PCR was consisted as follows，in the total reaction system with amount of 20 μL，there were 0.125 mmol?L-1 dNTPs，0.15 μL Taq DNA polymerase,1.0 μmol?L-1 primers，0.625 mmol?L-1 Mg2+，40 ng template DNA，respectively.The optimal annealing temperature was 51 ℃.The findings suggested that the products′ bands were clear with high stability.

References:

 

[1]倪志诚，西藏经济植物[M].北京:科学技术出版社，1990:308-310.

[2]李维林，蒋振军，蒋续银.粉枝莓资源开发利用研究[J].中国水土保持，1994(6)：37-39.

[3]康淑荷.粉枝莓根挥发油化学成分研究[J].西北民族大学学报(自然科学版)，2007，28(65)：27-29.

[4]康淑荷，师永清，杨彩霞.粉枝莓根中的三萜及甾体化合物[J].中药材，2008，31(11)：1669-1671.

[5]康淑荷，郑尚珍.粉枝莓中的两种新黄酮成分[J].药学学报，2007，42(12)：1288-1291.

[6]COLLARD B C Y，MACKILL D J.Start codon targeted(SCoT) polymorphism：A simple，novel DNA marker technique for generating gene-targeted markers in plants[J].Plant Mol Biol Rep，2009，27:86-93.

[7]韩国辉,汪卫星,向素琼,等.多倍体枇杷SCoT分析体系的建立与优化[J].果树学报，2011，28(3):433-437.

[8]张君玉,郭大龙,龚莹,等.葡萄目标起始密码子多态性反应体系的优化[J].果树学报,2011,28(2)：209-214.

[9]和世玉,牛建新,马建江.梨SCoT-PCR反应体系的优化及‘库尔勒香梨’营养系变异鉴定[J].果树学报,2016，33(11):1337-1346.

[10]李金璐,王硕,于婧,等.一种改良的植物DNA提取方法[J].植物学报，2013，48(1):72-78.

[11]李晓晖,黎颖菁,黄荣韶,等.凉粉草遗传多样性的SCoT和ISSR分析[J].西南农业学报,2012,25(5)：1834-1840.

[12]姜小凤,高燕会,童再康,等.黄春红石蒜属植物SCoT-PCR 反应体系构建及优化[J].浙江农林大学学报，2013，30(3)：444-452.

[13]秦国新,何桥,梁国鲁,等.草莓属植物SCoT分析体系的建立及优化[J].果树学报，2012(3):393-397.

[14]陈大霞,张雪,王钰,等.应用SCoT标记分析玄参种质资源的遗传多样性[J].中国中药杂志,2012,37(16)：2368-2372.

[15]蔡元保,杨祥燕,陈显国,等.澳洲坚果SCoT反应体系的建立及应用[J].热带亚热带植物学报,2013,21(3)：253-258.

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Last Update: 2017-03-22