|Table of Contents|

Cloning and Sequence Analysis of Two WD40 Transcription Factors in Ginkgo biloba L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年18
Page:
100-108
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of Two WD40 Transcription Factors in Ginkgo biloba L.
Author(s):
FENG Ru; LI Zehong; YUAN Honghui; CHENG Hua; CHENG Shuiyuan
School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan，Hubei 430023
Keywords:
Ginkgo biloba L.; flavonoid; WD40 transcription factor; gene cloning
PACS:
-
DOI:
10.11937/bfyy.20170279
Abstract:
WD40 transcription factor,also known as WDR (WD repeat),was an ancient family of proteins.It could participate in a few genes′ co-expression in the plant flavonoid secondary metabolic pathway,effectively improved the contents of flavonoids.This study used triennial gingko trees as materials,used RT-PCR and RACE-PCR technology explored the sequences of WD40,in order to understand the molecular mechanism and transcriptional regulation mechanisms of flavonoid metabolism in Ginkgo.The results showed that GbWD401 contained a 2 313 bp ORF encoding 770 amino acid residues,and GbWD402 contained a 1 605 bp ORF encoding 534 amino acid residues.The predicted protein sequences of GbWD401 and GbWD402 genes both contained the typical WD40 structural domain.GbWD401 shared 49% similarity with AtWD401 of Amborella trichopoda, GbWD402 shared 78% similarity with AtWD402 of Amborella trichopoda.Phylogenetic tree analysis showed that GbWD401 was grouped into the same clade with AtWD401 of Amborella trichopoda and NnWD40 of Nelumbo nucifera,while GbWD402 was grouped into the other clade with AtWD402 of Amborella trichopoda.

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Last Update: 2017-10-06