|Table of Contents|

Tissue Culture and Rapid Propagation of Ledebouria socialis (Liliaceae)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年21
Page:
104-108
Research Field:
Publishing date:

Info

Title:
Tissue Culture and Rapid Propagation of Ledebouria socialis (Liliaceae)
Author(s):
JIA Huimei; LI Kang; KANG Silu; CAI Yanni; LI Huining
College of Biological Science and Engineering,Shaanxi Sci-Tech University/Shaanxi Province Key Laboratory of Bio-resources,Hanzhong,Shaanxi 723001
Keywords:
Ledebouria socialis (Baker) Jessop; explants; tissue culture; rapid propagation
PACS:
-
DOI:
10.11937/bfyy.20170245
Abstract:
The leaf blade，petiole and bulbaceous from Ledebouria socialis (Baker) Jessop were used as explants,which were cultured on MS media supplemented with different hormones to establish a rapid propagation system.The results confirmed a reliable method,which could be used for mass propagation of L.socialis at the commercial level.The results showed that different explants could induce callus,while those from leaf blades were rather successful.The optimal medium for callus induction was MS+6-BA 2.0 mg?L-1+NAA 0.5 mg?L-1,and the induction percentage was 96.3%-98.8%;MS+6-BA 1.0 mg?L-1+NAA 0.1 mg?L-1 were suitable for differentiation of adventitious buds,and with 91.1% in induction percentage.The medium MS+NAA 0.2 mg?L-1 was proper for forming roots,and rooting rate could be up to 89.5%.Transplanting experiment showed that the tissue cultured seedling grew with more than 86.7% survival rate.

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Last Update: 2017-11-14