|Table of Contents|

Three-wavelength Ultraviolet Spectrophotometric Determination of Total Flavonoes  From Cordyceps kyushuensis Extracted by Supercritical Fluid Method(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年02
Page:
132-135
Research Field:
Publishing date:

Info

Title:
Three-wavelength Ultraviolet Spectrophotometric Determination of Total Flavonoes  From Cordyceps kyushuensis Extracted by Supercritical Fluid Method
Author(s):
ZHU Jianmin1; 2; ZHANG Guoying3; DOU Peipei1; GENG Yanping1; FENG Jing1; LING Jianya1
(1.State Key Laboratory of Microbial Technology，Shandong University，Jinan，Shandong 250100；2.Department of Marine Economy，Shandong Light Industry Engineering College，Qingdao，Shandong 266112；3.College of Pharmacy,Shandong University of Traditional Chinese Medicine，Jinan，Shandong 250014)
Keywords:
Cordyceps kyushuensis; flavone; three-wavelength ultraviolet spectrophotometry; supercritical fluid extraction
PACS:
-
DOI:
10.11937/bfyy.201702032
Abstract:
Cordyceps kyushuensis was used as test material，three-wavelength ultraviolet spectrophotometry was used for determining the content of total flavones in Cordyceps kyushuensis which was extracted by Supercritical fluid method (SFE).The results showed that regression equation of ΔA rutin standard concentration was obtained：y=0.025 3x-0.090 4 with a relation coefficient r2=0.999 7.Rutin concentrations in 16-56 μg?mL-1 with ΔA obeyed the linear relation measured at wavelength λ1=302 nm，λ2=272 nm and λ3=244 nm.It indicated that the method had a good stability.The recovery was 94.80%-97.75%；the relative standard deviation was less than 0.67% (n=6).The content of total flavones extracted from Cordyceps kyushuensis by SFE was 1.89 mg?g-1.

References:

 

[1]CRITCHFIELD J W，COLIGAN J E，FOLKS T M.Casein kinase Ⅱ is a selective target of HIV-1 transcriptional inhibitors[J].Proc Natl Acad Sci，1997，94(12)：6110-6115.

[2]CACCIOLA F，JANDERA P，HAJDU〖KG-1.8mm〗′〖KG1.5mm〗 Z，et al.Comprehensive two-dimensional liquid chromatography with parallel gradients for separation of phenolic and flavone antioxidants[J].J Chromatogr A,2007，1149:73-87.

[3]THOMAS W.Methoxylated flavones，a superior cancer chemopreventive flavonoid subclass[J].Semin Cancer Biol,2007，17(5)：354-362.

[4]郭英兰，李春燕.中国一新记录种-九州虫草[J].菌物系统,2000，19(2):296.

[5]丁明玉，赵纪萍，李擎阳.贯叶金丝桃提取物中总黄酮的测定方法[J].分析试验室，2001，20(6)：45-47.

[6]de RIJKE E，ZAFRA-GOMEZ A.Determination of isoflavone glucoside malonates in Trifolium pratense L.(red clover) extracts：quantification and stability studies[J].J Chromatogr A，2001，932:55-64.

[7]LAI J P，LIM Y H，SU J，et al.Identification and characterization of major flavonoids and caffeoylquinic acids in three Compositae plants by LC/DAD-APCI/MS[J].J Chromatogr B，2007,848(2):215-225.

[8]符泰然,程晓舫,范学良,等.三波长测温中波长选取原则的理论分析[J].光谱学与光谱分析，2005,25(2)：166-170.

[9]回瑞华,侯冬岩,关崇新,等.三波长分光光度法测定沱茶中茶多酚的含量[J].光谱学与光谱分析，2005,25(2)：266-269.

[10]尚永辉,孙佳娟,刘彬,等.三波长紫外分光光度法测定葡萄籽中黄酮含量[J].分析科学学报，2013，29(5):706-708.

[11]鞠兴荣，袁建，汪海峰.三波长紫外分光光度法测定大豆异黄酮含量的研究[J].食品科学,2001,22(5):46-48.

Memo

Memo:
-
Last Update: 2017-03-22