|Table of Contents|

Cloning and Bioinformatics Analysis of DOF Protein Gene in Brassica napus L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年14
Page:
18-24
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatics Analysis of DOF Protein Gene in Brassica napus L.
Author(s):
YIN MingzhiHU Yan
(College of Biology and Agricultural Science & Technology,Zunyi Normal University,Zunyi,Guizhou 563006)
Keywords:
Brassica napus L.Dof protein genesilico cloningbioinformatics
PACS:
-
DOI:
10.11937/bfyy.20170162
Abstract:
Dof transcription factor is a kind of plant specific transcription factor,which contains an unique single zinc finger domain,and plays an important role in the growth and development of plants.In this study,a cDNA gene sequence with Zf-Dof domain was cloned by silico cloning and RT-PCR in Brassica napus.The results showed that the gene was a hydrophilic protein,containing an open reading frame of 1 284 bp,encoding a total of 427 amino acids with a molecular weight of 46.9 kDa and an isoelectric point of 8.86.Sequence alignment analysis showed that it had high homology with Arabidopsis CDF3 gene,which might have similar functions.

References:

 

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Last Update: 2017-07-25