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Isolation and Identification of Endophytic Fungi From Liparis japonica and Screening for Antimicrobial Activities(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年01
Page:
126-130
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Endophytic Fungi From Liparis japonica and Screening for Antimicrobial Activities
Author(s):
DING Rui12LI Mengkai1REN Guangyu1JIN Yuqi1ZHANG Haoqi1CHEN Xuhui1
(1.College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang,Liaoning 110866;2.College of Land and Environment,Shenyang Agricultural University,Shenyang,Liaoning 110866)
Keywords:
endophytic fungiantimicrobial activityLiparis japonicaOrchidaceae
PACS:
-
DOI:
10.11937/bfyy.201701027
Abstract:
Liparis japonica species (Orchidaceae) was used as test material,the endophytic fungi from the roots were isolated by routine sterile culture techniques and then their antimicrobial activities on several common crop pathogens were studied by using plate cultivation methods.The results showed that a total of 43 isolates of endophytic fungi were obtained.They were classified into seven different genus according to their morphological characters,and Rhizoctonia genus was the dominant genus.Four isolates (9.3% of the total isolates obtained) showed antimicrobial activities on the test crop pathogens,and the antimicrobial activities mainly focused on Sclerotimia sclerotiorum.The phylogenetic analysis of ITS sequences indicated these four isolates belonged to Fusarium solani,Cylindrocarpon sp.,Lecanicillium sp.and Tulasnella sp.,respectively.

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Last Update: 2017-03-17