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Optimizing of Different RNA Isolation Methods for Lycium Linn Leaves(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年01
Page:
114-117
Research Field:
Publishing date:

Info

Title:
Optimizing of Different RNA Isolation Methods for Lycium Linn Leaves
Author(s):
FU Haihui1; 2; ZHANG Bo3; CHENG Hui1; 2; 3; LI Yanlong3; CAO Youlong3; ZHOU Jun1; 2
（1.Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province，Kunming，Yunnan 650224；2.Forestry College，Southwest Forestry University，Kunming，Yunnan 650224；3.Center of Chinese Wolfberry Engineering and Technology，Yinchuan，Ningxia 750002)
Keywords:
wolfberry; leaves; RNA; optimized guanidinium isothiocyanate method
PACS:
-
DOI:
10.11937/bfyy.201701025
Abstract:
The performances of four extraction methods，including CTAB，guanidinium isothiocyanate,Trizol and SDS were compared to select the suitable method for total RNA extraction from Lycium Linn leaves.The results showed that RNA could not be extracted perfectly by original methods and RNA non-clearness as well as impurity，but the optimizing guanidinium isothiocyanate method was perfect and total RNA could be used for RT-PCR.

References:

 

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Last Update: 2017-03-16