|Table of Contents|

Study on Content Determination of 6-ketone Steroidal  Saponins From Smilax scobinicaulis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年01
Page:
107-113
Research Field:
Publishing date:

Info

Title:
Study on Content Determination of 6-ketone Steroidal  Saponins From Smilax scobinicaulis
Author(s):
QI Yawen1; WANG Rong1; ZHAO Qiulin2; ZHANG Cunli1
（1.College of Life Science，Northwest Agricultural and Forestry University，Yangling，Shaanxi 712100；2.Inspection & Testing Center，Linyi，Shandong 276300）
Keywords:
Smilax scobinicaulis C.H.Wright; 6-ketone type steroidal saponins; UV-VIS spectrophotometry; CCD-RSM
PACS:
-
DOI:
10.11937/bfyy.201701024
Abstract:
To develop a method for the determination the contents of 6-ketone steroidal saponins from Smilax scobinicaulis C.H.Wright，using LG-C as standard substance，different chromogenic agents，the optimal UV-VIS spectrophotometry and the optimize the chromogenic condition were studied.The results showed that the proposed method was anisaldehyde sulphuric acid Ⅱ in the circumstances，and，the maximum absorption wavelength was 429 nm.The optimal temperature was 82.4 ℃，the best reaction time was 24 min，and 1.2 mL chromogenic agent was fitted.The calibration curve of LG-C showed good linearity in the range of 7.51-50.90 μg，with r=0.996 9.The recovery was detected to be 103.37%，with RSD being 2.2%，respectively.The established colorimetric method provided sensitive，specific and accurate for determination of 6-ketone type steroidal saponins in Smilax scobinicaulis C.H.Wright.

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Last Update: 2017-03-16