|Table of Contents|

Leaf Transcriptome Sequencing and Bioinformatics Analysis of Lowbush Blueberry Variety ‘Blomidon’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年01
Page:
101-107
Research Field:
Publishing date:

Info

Title:
Leaf Transcriptome Sequencing and Bioinformatics Analysis of Lowbush Blueberry Variety ‘Blomidon’
Author(s):
WANG Yunsheng1; WEN Guangqin2; NIE Fei3
（1.School of Environment and Life Sciense，Kaili University，Kaili，Guizhou 556011；2.Botanic Institute of Guizhou Province，Guiyang，Guizhou 550004；3.Biological Institute of Guizhou Province，Guiyang，Guizhou 550000）
Keywords:
bioinformatics; lowbush blueberry; transcriptome; high-throughout sequencing
PACS:
-
DOI:
10.11937/bfyy.201701023
Abstract:
In the hope of increasing the understanding of molecular mechanism of the growth and development of Vaccinium angustifolium，and establishing the foundation for the molecular marker assisted breeding of dwarf blueberry，cultivar ‘Blomidon’ of dwarf blueberry was selected as test material，and its young leave transcriptome was sequenced by the second sequencing technology and then was made bioinformatics analysis，the aim was to study the functional genomic information of lowbush blueberry.The results showed that,total about 1 G clean reads could been assembled into 41 929 Unigenes.Among them，Unigenes were involving 45 kinds metabolism and physiological function of biological process，cellular component and molecular function according to the annotation results of dataset GO；19 149 Unigenes could been annotated by KOG dataset，which were involving in 25 metabolism pathways；13 072 Unigenes cauld been annotated by KEGG dataset，which were involving in five major categories，18 middle categories，and 129 metabolism pathways；30 738 Unigenes could been annotated by dataset Nr，which were mapping into 32 949 code regions of protein.In the meanwhile，bioinformatics analysis showed that 884 Unigenes were the transcript factor candidate of 55 families，and 2 261 Unigenes were R gene candidate of 18 families，total of 6 591 SSR loci were detected，of which 2 base repeat SSR accounted for about 70%.

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Last Update: 2017-03-16