|Table of Contents|

Constructing of Expressing Vector and Identifying of Binding Activity of AmDREB2.1 in Ammopiptanthus mongolicus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年01
Page:
95-100
Research Field:
Publishing date:

Info

Title:
Constructing of Expressing Vector and Identifying of Binding Activity of AmDREB2.1 in Ammopiptanthus mongolicus
Author(s):
XIA BolinGAO FeiWEI ShanjunWANG XueZHOU Yijun
(College of Life and Environmental Sciences,Minzu University of China,Beijing 100081)
Keywords:
Ammopiptanthus mongolicusAmDREB2.1plant expression vectoryeast one-hybrid vectorbinding activity
PACS:
-
DOI:
10.11937/bfyy.201701022
Abstract:
Ammopiptanthus mongolicus was used as material,the AmDREB2.1 transformation to tobacco was investigated via expression vector construction and leaf disc method,and the binding activity of AmDREB2.1 was studied with Gateway technique and the yeast one-hybrid assay.The results showed that pPZP212-AmDREB2.1 expression vector was constructed with BamH I and Sac I,and then successfully transformed to the tobacco.The pDEST22-AmDREB2.1,the yeast one-hybrid vector,was constructed,and then were converted into two yeast strains YH4271-mDRE and YH4271-wDRE,respectively.Positive clones were obtained with the ‘Trp-His-Ade-’selective media (the concentration of 3-AT was 0 mmol?L-1 and 20 mmol?L-1),and the activity of β-galactosidase was detected and it indicated that AmDREB2.1 could bind to DRE cis-element.

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Last Update: 2017-03-16