|Table of Contents|

Induction of Callus and Regeneration of Cucurbita maxima Duch.in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年15
Page:
42-47
Research Field:
Publishing date:

Info

Title:
Induction of Callus and Regeneration of Cucurbita maxima Duch.in vitro
Author(s):
LU Xiaoxiao; GUO Weitao; ZHOU Junguo; JIANG Yi; JIANG Lina; CHEN Xuejin
(School of Horticulture Landscape Architecture,Henan Institute of Science and Technology,Xinxiang,Henan 453003)
Keywords:
Cucurbita maxima; explants; callus; regeneration
PACS:
-
DOI:
10.11937/bfyy.20165033
Abstract:
The hypocotyls,cotyledons,true leaves and stem segments of C.maxima ‘Beiguan’ inbred seedlings were used as explants,regenenration in vitro method was used to study the effects of different kinds of hormones on the development of callus in subculture,and to explore the regeneration of callus in vitro respectively for constructing high efficient regeneration system by callus way of pumpkin.The results showed that the callus could be induced from the hypocotyls,cotyledons,true leaves and stem segments of C.maxima ‘Beiguan’ inbred seedlings,and cotyledons and stem as explants were easier to induce callus than hypocotyls and true leaves.MS+1.0 mg?L-1 6-BA（or 2.0 mg?L-1 KT）+0.2 mg?L-1 NAA medium was better in callus subculture by obtaining better quality of the callus.In the induction of callus regeneration culture,none the adventitious bud was inducted from all callus explants with different hormone combinations,only part of the callus differentiated into adventitious roots.

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Last Update: 2017-08-03