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Seperation,Identification and Culture Conditions of Cortinarius sp.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年14
Page:
169-174
Research Field:
Publishing date:

Info

Title:
Seperation,Identification and Culture Conditions of Cortinarius sp.
Author(s):
LI Min12YAO Qingzhi23WEI Jie24LIU Yang23LI Yizhen2ZHANG Xuan2
(1.College of Life Science and Technology,Inner Mongolia Normal University,Huhhot,Inner Mongolia 010022;2.Inner Mongolia Hesheng Institute of Ecological Sciences & Technology,Huhhot,Inner Mongolia 011517;3.College of Life Sciences,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia 010018;4.Forestry College,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia 010018)
Keywords:
Cortinarius sp.separationidentificationculture conditions
PACS:
-
DOI:
10.11937/bfyy.20165005
Abstract:
A strain of Cortinarius sp.was isolated from a wild mushroom fruiting body under Picea crassifolia forest,from Helan Mountain,and was identified based on its morphology and ITS sequence analysis.The optimum culture conditions,i.e.medium,temperature,pH and the effects of extraction from the coniferous,branches and roots of Picea crassifolia on the mycelium growth were investigated.The results showed that the strain manifested relatively high sequence similarity with Cortinarius rufoolivaceus (Canada) samples based on polymeric analysis of ITS sequences,with similarity of 98%.Combined with its morphological characteristics,it was therefore identified as Cortinarius sp.LM-1.Culture conditions showed that the optimum temperature was 15-20 ℃,and the optimum pH was 5.5-6.0.The branch and needle extraction of the host plant Picea crassifolia had significant roles in promoting the mycelium growth,enhanced the mycelial dry weight ((8.62±0.27) mg and (6.93±0.27) mg) with the value of 45.85% and 17.26% comparing with the control group.

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Last Update: 2017-07-26