|Table of Contents|

Promoting Effect of Endophytic Diazotrophs From Bermudagrass on  Zoysia japonica Steud(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年13
Page:
113-118
Research Field:
Publishing date:

Info

Title:
Promoting Effect of Endophytic Diazotrophs From Bermudagrass on  Zoysia japonica Steud
Author(s):
WU Xin1; 2; LI Fengjiao1; 2; LIU Tianzeng1; 2; ZHANG Juming1; 2
(1.College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,Guangdong 510642;2.Engineering Research Center of Grassland Science,South China Agricultural University,Guangzhou,Guangdong 510642)
Keywords:
endophyte diazotroph; inoculation; Zoysia japonica Steud; promoting effect
PACS:
-
DOI:
10.11937/bfyy.20164941
Abstract:
Taking Zoysia japonica Steud as test materials,the endophyte diazotroph QD-3 was isolated from bermudagrass and made into two different concentration bacteria liquid(1×103 cfu?mL-1 and 1×106 cfu?mL-1).After soaking Zoysia japonica Steud stems with similar growth for 4 hours and 16 hours respectively,the potting test began.When turf established,the turf color,tillers number,aboveground biomass,underground biomass and total nitrogen were evaluated to study the promoting effect of endophytic diazotrophs on zoysia grass.In the meanwhile,the versatility between different grass species could also be explored.The results showed that soaking under the concentration of 1×106 cfu?mL-1 for 4 hours had the most promoted tillers number;soaking under the concentration of 1×106 cfu?mL-1 for 16 hours had well-influenced turf color;soaking under the concentration of 1×106 cfu?mL-1 for 16 hours showed the most improved aboveground biomass while soaking under the concentration of 1×103 cfu?mL-1 for 4 hours had greater underground biomass.Soaking under the concentration of 1×106 cfu?mL-1 for 16 hours had the best promotion in total nitrogen of Zoysia japonica Steud.In conclusion,soaking under the concentration of 1×106 cfu?mL-1 for 16 hours had the best promoting effect on Zoysia japonica Steud.

References:

 

[1]刘天增,毛中伟,李凤娇,等.狗牙根内生固氮菌的分离鉴定[J].草业科学,2014,31(7):1254-1260.

[2]安千里,李久蒂.植物内生固氮菌[J].植物生理学通讯,1999,35(4):265-272.

[3]MONTA〖AKN~〗EZ A,ABREU C,GILL P R,et al.Biological nitrogen fixation in maize (Zea mays L.) by 15N isotope-dilution and identification of associated culturable diazotrophs[J].Biology and Fertility of Soils,2009,45(3):253-263.

[4]罗明,卢云.植物内生固氮菌研究进展[J].微生物学杂志,2005,25(1):82-88.

[5]许褆森.内生固氮菌研究进展[J].安徽农业科学,2008,36(12):4828-4830.

[6]JAMES E K.Nitrogen fixation in endophytic and associative symbiosis[J].Field Crops Research,2000,65(2-3):197-209.

[7]冯宏,郭彦彪,林日强,等.类芦和狗牙根内生固氮菌初步研究[J].热带亚热带植物学报,2009,17(5):465-470.

[8]谭志远,傅琴梅,彭桂香,等.青香茅和五节芒内生固氮菌的分离与生理生化鉴定[J].应用与环境生物学报,2013,19(4):643-649.

[9]秦宝军,罗琼,高淼,等.小麦内生固氮菌分离及其ACC脱氨酶测定[J].中国农业科学,2011,45(6):1066-1073.

[10]孙建光,罗琼,高淼,等.小麦,水稻,玉米,白菜,芹菜内生固氮菌及其系统发育[J].中国农业科学,2012,45(7):1303-1317.

[11]孟宪法,隆小华,康健,等.菊芋内生固氮菌分离,鉴定及特性研究[J].草业学报,2011,20(6):157-163.

[12]欧阳雪庆,罗霆,杨丽涛,等.甘蔗内生固氮菌液浸种对甘蔗生长前期氮代谢相关酶活性的影响[J].广西农业科学,2010,41(5):416-418.

[13]王华荣,彭桂香,张国霞,等.糖蜜草(Melinis minutiflora Beauv.)内生固氮菌分离鉴定[J].生态学报,2006,26(8):2566-2571.

[14]李凤娇,毛中伟,黎瑞君,等.杂交狗牙根内生固氮菌的接种效果[J].草业科学,2013,30(12):1953-1959.

[15]李倍金,罗明,周俊,等.几种禾草内生固氮菌的分离及固氮活性测定[J].草业学报,2008,17(5):37-42.

[16]南志标.内生真菌对布顿大麦草生长的影响[J].草业科学,1996,13(1):16-18.

[17]赵小蓉,林启美.微生物解磷的研究进展[J].土壤肥料,2001(3):1-11.

[18]南京农学院.土壤农化分析[M].北京:农业出版社,1980.

[19]王坚,刁治民,徐广,等.植物内生菌的研究概况及其应用[J].青海草业,2008,17(1):24-28.

[20]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.

[21]布坎南R E,吉本斯N E.伯杰氏细菌鉴定手册[M].北京:科学出版社,1984.

[22]田宏,张徳罡,姚拓,等.禾本科草坪草固氮菌株筛选及部分特性初步研究[J].中国草地,2005,27(5):47-52.

[23]孙吉雄.草坪学[M].2版.北京:中国农业出版社,2003:355-370.

[24]戴宏林,吴小骏.用凯氏定氮法测定植物干样品中的氮含量[J].江苏农学院学报,1995,16(3):70.

[25]席琳乔,姚拓,张德罡.固氮菌对燕麦不同生育期促生作用的研究[J].草业学报,2007,16(3):38-42.

[26]夏觅真,马忠友,曹媛媛,等.棉花根际固氮菌、解磷菌及解钾菌的相互作用[J].中国微生态学杂志,2010(2):102-105.

[27]张丽梅,方萍,朱日清.禾本科植物联合固氮研究及其应用现状展望[J].应用生态学报,2004,15(9):1650-1654.

[28]马天瑞,蓝祖庆.苜蓿根瘤菌对燕麦促生作用的研究初报[J].安徽农学通报,2010,16(23):6.

[29]张晓霞,王平.对水稻有促生作用的紫云英根瘤菌筛选初报[J].土壤肥料,2001(6):30-33.

[30]胡桂萍,郑雪芳,尤民生,等.植物内生菌的研究进展[J].福建农业学报,2010,25(2):226-234.

[31]BURD G I,DIXON D G,GLICK B R.Plant growthpromoting bacteria that decrease heavy metal toxicity in plants[J].Canadian Journal of Microbiology,2000,46(3):237-245.

[32]汪军,黄俊生.植物内生菌研究技术及应用研究进展[J].广西热带农业,2008,18(5):18.

[33]CHANWAY C P,SHISHIDO M,NAIRN J,et al.Endophytic colonization and field responses of hybrid spruce seedlings after inoculation with plant growth-promoting rhizobacteria[J].Forest Ecology and Management,2000,133(1):81-88.

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Last Update: 2017-07-18