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Enhancement of Polysaccharide and Alkaloid Accumulation inProtocorm-like-bodies of Dendrobium candidum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年15
Page:
117-123
Research Field:
Publishing date:

Info

Title:
Enhancement of Polysaccharide and Alkaloid Accumulation inProtocorm-like-bodies of Dendrobium candidum
Author(s):
WANG Hongqiu; PIAO Xuanchun; JIANG Xiaolong; LIU Guanfu; LIAN Meilan
(Agricultural College,Yanbian University,Yanji,Jilin 133002)
Keywords:
suspension culture; pH; elicitor; chitosan; pullulan
PACS:
-
DOI:
10.11937/bfyy.20164892
Abstract:
Dendrobium candidum Lindl.protocorm-like-bodies (PLBs) was used as experiment material,for optimizing the protocorm-like-bodies culture and enhancing the accumulation of effective compounds,the effect of different medium pH on the biomass and effective compound accumulation of PLBs were studied.At the same time,different concentrations of elicitor (chitosan,pullulan polysaccharide) and two kinds of elicitors mixture were added to the culture medium at the early stages of cultivation,the culture without the addition of the elicitor was used as control,and then the biomass and effective compound accumulation of PLBs were determined.The results showed that the growth of PLBs were the best when the pH of the medium was 5.8,and the production of polysaccharide (4.93 g?L-1) and alkaloid (3.15 mg?L-1) reached the highest level.The separate treatment of 30 mg?L-1 chitosan and 200 mg?L-1 pullulan which were beneficial to the accumulation of polysaccharide and alkaloid in PLBs.However,when the chitosan concentration was adjusted to 15 mg?L-1 and the concentration of pullulan was adjusted to 100 mg?L-1,the accumulation of polysaccharide and alkaloid in PLBs were significantly increased,and the polysaccharide content reached 322.8 mg?g-1 DW,the production reached 6.75 g?L-1,while the alkaloid content reached 242.7 μg?g-1 DW,the production reached 5.07 mg?L-1.Therefore,polysaccharide and alkaloid accumulation in D.candidum PLBs could be effectively promoted by control the culture conditions and which could be used as a new material resource in production of Dendrobium candidum production.

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Last Update: 2017-08-04