|Table of Contents|

Cloning and Expression Analysis for Copper Tolerance Related  Gene CsLecRK in Cucumber(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年13
Page:
14-21
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis for Copper Tolerance Related  Gene CsLecRK in Cucumber
Author(s):
ZHAO Jinfeng12TIAN Chunyu12GUO Dongxue12WU Tao12
(1.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture,Northeast Agricultural University,Harbin,Heilongjiang 150030;2.College of Horticultural and Landscape Architecture,Northeast Agricultural University,Harbin,Heilongjiang 150030)
Keywords:
cucumbercopper tolerance related geneCsLecRKexpression analysis
PACS:
-
DOI:
10.11937/bfyy.20164582
Abstract:
Cucumber was used as test material.The mechanism of copper (Cu) tolerance in cucumber was investigated,a candidate Cu tolerance related gene,named lectin receptor kinase (CsLecRK), was cloned and analyzed by using gene cloning and bioinformatics methods based on expression pattern and conservative of genes response to Cu.The results showed that full-length cDNA of CsLecRK was 2 121 bp and encoded a protein of 706 amino acids with predicted molecular mass of 78.36 kDa and a pI of 7.76.Both the results of NCBI Blastp and phylogenetic tree based on LecRK proteins showed that CsLecRK protein had the highest homology with that of muskmelon (95%),the evolutionary distance was the shortest.The result of qRT-PCR showed that CsLecRK was significantly down-regulated under Cu stress.

References:

 

[1]张立军,刘新.植物生理学[M].2.北京:科学出版社,2011.

[2]杨丽丽.铜胁迫对甜菜幼苗生长和光合特性的影响[D].济南:山东师范大学,2013.

[3]胡筑兵,陈亚华,王桂萍,.铜胁迫对玉米幼苗生长、叶绿素荧光参数和抗氧化酶活性的影响[J].植物学通报,2006,23(2):129-137.

[4]林义章,张淑媛,朱海生,.铜胁迫对小白菜叶肉细胞超微结构的影响[J].中国生态农业学报,2008,16(4):948-951.

[5]王瑞刚,唐世荣,郭军康,.铜胁迫对高丹草和紫花苜蓿生长和光合特性的影响[J].生态环境学,2010,19(12):2922-2928.

[6]XIONG Z T,LIU C,GENG B.Phytotoxic effects of copper on nitrogen metabolism and plant growth in Brassica pekinensis Rupr.[J].Ecotoxicology and Environmental Safety,2006,64273-280.

[7]肖志华,张义贤,张喜文,.外源铅、铜胁迫对不同基因型谷子幼苗生理生态特性的影响[J].生态学,2012,32(3):889-897.

[8]刘小红.九华铜矿重金属污染调查及耐铜植物的筛选耐性机理研究[D].合肥:安徽农业大学,2005.

[9]RAMOS M C.Metals in vineyard soils of the penedes area (NE Spain) after compost application[J].Journal of Environmental Management,2006,721-7.

[10]黄永东,黄永川,于官平,.蔬菜对重金属元素的吸收和积累研究进展[J].长江蔬菜,2011(10):1-6.

[11]JANAS KZIELINSKA-TOMASZEWSKA JRYBACZEK Det al.The impact of copper ions on growthlipid peroxidationand phenolic compound accumulation and localization in lentil(Lens culinaris Medic.) seedlings[J].Journal of Plant Physiology,2010,167270-276.

[12]王松华,杨志敏,徐朗莱.植物铜素毒害及其抗性机制研究进展[J].生态环境,2003,12(3):336-341.

[13]孙权,何振立,杨肖娥,.铜对小白菜的毒性效应及其生态健康指标[J].植物营养与肥料学报,2007,13(2):324-330.

[14]NISHIGUCHI N M,YOSHIDA Y K,SUMIZONO S T,et al.A receptor-like protein kinase with a lectin-like domain from lombardy poplarGene expression in response to wounding and characterization of phosphorylation activity[J].Mol Gen Genomics,2002,267:506-514.

[15]RIOU C,HERV C,PACQUIT V,et al.Expression of an Arabidopsis lectin kinase receptor gene,lecRK-a1,is induced during senescence,wounding and in response to oligogalacturonic acids[J].Plant Physiol Biochem,2002,40:431-438.

[16]HE X J,ZHANG Z G,YAN D Q,et al.A salt-responsive receptor-like kinase gene regulated by the ethylene signaling pathway encodes a plasma membrane serine/threonine kinase[J].Theor Appl Genet,2004,109:377-383.

[17]FUJIWARA THIRAI M YCHINO M,et al.Effects of sulfur nutrition on expression of the soybean seed storage protein genes in transgenic Petunia[J].Plant Physiology,1992,99(1):263-268.

[18]LIVAK K JSCHMITTGEN D T.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J].Methods,2001(25)402-408.

[19]HERVE CDABOS PGALAUD J Ret al.Characterization of an Arabidopsis thaliana gene that defines a new class of putative plant receptor kinases with an extracellular lectin like domain[J].J Mol Biol,1996,258:778-788.

[20]JOSHI ADANG H QVAID Net al.Pea lectin receptor-like kinase promotes high salinity stress tolerance in bacteria and express in response to stress in planta[J].Glycoconjugate Journal2010,27(1):133-150.

[21]HAFFANI Y ZSILVA N FGORING D R.Receptor kinase signalling in plants[J].Canadian Journal of Botany,2004,82(1)1-15.

[22]BARRE AHERV ECHRISTINEet al.Lectin receptor kinases in plants[J].Critical Reviews in Plant Sciences,2002(21):379-399.

[23]XIN ZWANG AYANG Get al.The Arabidopsis A4 subfamily of lectin receptor kinases negatively regulates abscisic acid response in seed germination[J].Plant Physiol,2009,149:434-444.

[24]SONIA M.PHILLIPS I ADUBERY H V H.Identification and molecular characterisation of a lectin receptor-like kinase (GhLecRK-2) from cotton[J].Plant Mol Biol Rep,2013,31:9-20.

[25]JOHANNES AHOFBERGERDAVID L,et al.A complex interplay of tandem-and whole-genome duplication drives expansion of the L-type lectin receptor kinase gene family in the Brassicaceae[J].Genome Biol Evol,2015,7(3):720-734.

[26]JIANG ZGE S.RLP1.1a novel wheat receptor-like protein geneis involved in the defence response against Puccinia striiformis f.sp.tritici[J].Journal of Experimental Botany2013,64(12):3735-3746.

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Last Update: 2017-07-17