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Cloning of SsPIN2 Gene From Sinningia speciosa and Its Expression in Different Tissues(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年24
Page:
88-91
Research Field:
Publishing date:

Info

Title:
Cloning of SsPIN2 Gene From Sinningia speciosa and Its Expression in Different Tissues
Author(s):
XU Weiping1LIU Fengjuan2BI Chunxiao2HU Xin2XU Quanle2JIANG Jinglong1
(1.School of Biological Science and Engineering,Shaanxi Sci-tech University,Hanzhong,Shaanxi 723001;2.College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100)
Keywords:
Sinningia speciosaSsPIN2 genegene cloningexpression analysis
PACS:
-
DOI:
10.11937/bfyy.201624022
Abstract:
Sinningia speciosa were used as materials,and 898 bp cDNA in length of SsPIN2 gene was isolated.BLAST and phylogenetic trees via neighbor-joining (NJ) method were used to analysis sequence.And semi-quantitative RT-PCR was used to investigate the different expression level of SsPIN2 gene in different tissues of leaf,shoot tips,root,stem,petal and calyx.The results indicated that SsPIN2 shared a high sequence identity with CsPIN2 and PsPIN2. SsPIN2 was expressed in all of the above tissues and relatively higher in shoot tips,root,stem and calyx and relatively lower in leaf and petal.

References:

 

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Last Update: 2017-02-13